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Information × Registration Number 0211U001833, 0106U000336 , R & D reports Title To work out technology of receipt of biological preparation on the basis of barrel cages of embryonic liver for the correction of the system of immunohaematopoiesis popup.stage_title Head Svetlana A. Fisenko, Registration Date 07-02-2011 Organization National Scientific Center "Institute of Experimental and Clinical Veterinary Medicine" popup.description2 The object of study - the cells of fetal liver dogs. The purpose of the work - to develop technology for producing biological preparation based on fetal liver stem cells to correct the system of immunohaematopoiesis. Methods of research - biotechnology, bacteriological, clinical, biochemical, histological. The technology of cryopreservation of cells of embryonic liver of dogs is developed. Improved method for obtaining cells of embryonic liver dog, which allows to receive 120 million cells from the 1g in fetal liver tissue with viability of 88.2%, which is 1,5-2 times higher than with the traditional method of disaggregation of tissues. Developed a cryoprotective medium, which allows to save up to 82.2% of viable cells of fetal liver. A morphological analysis of suspensions of cells derived from the liver of embryos of different ages, and the features of their proliferation. It was established that the drug is based on suspensions of CEL contains undifferentiated blasts in the form of large round cells with large nuclei, as well as hepatocytes and cells of erythroid and lymphoid series. Optimum terms were neat for the receipt of cages of haematopoiesis row at cultivation іn vіtro. Shows the effect of different combinations of growth factors on cell cultures of embryonic liver dogs. Characteristics of the effectiveness of colony forming cells - precursors during the whole period of cultivation. The tests produced the drug on the basis of a suspension of fetal liver cells in dogs in various pathologic states. A positive effect of a biological product based on the CEL on the protective, regenerative and reparative processes in dogs with hepatic insufficiency. The influence of the cryopreserved cells on the system immunohaematopoiesis of animals. Established that the application of biological preparation based on the CEL in a dose of 40 thousand cells/kg of animal body weight prevented the development of immunosuppression and stops the development of anemic state in patients with dogs. Product Description popup.authors Акомьолкова Г.І. Келеберда М.І. Коваленко Л.В. Тукан І.В. Шутченко П.О. popup.nrat_date 2020-04-02 Close