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Information × Registration Number 0211U002629, 0108U001990 , R & D reports Title To study regularities of morphogenesis in culture of isolated anthers of tomato cultivated forms and interspecific hybrids germs in vitro for optimization of transgressive and introgressive breeding popup.stage_title Head Ivchenko Tatiana Vladimirovna, Registration Date 17-02-2011 Organization Institute of vegetable and melons Ukrainian Academy of Agrarian Sciences popup.description2 Morphogenesis is researched in the culture of the isolated fabrics and organs of tomato in vitro. The influence of exogenous conditions of development, namely stress factors of biotic and antibiotic origin. Purpose of researches: theoretical and experimental ground of the using of biotechnology methods in plant-breeding researches on creation and genetic stabilizing of initial material for the geterosis breeding of tomato. The new methodological approaches are developed to the creation of high-quality of new plant-breeding material of tomato by the way of association gaploinduction on the basis of androgenesis and cellular breeding on resistance to stress factors in the culture of isolated anthers, bringing in of embryonic plasma of incompatible species of tomato and mutant forms. Genotypes are exposed with gaploinductional properties. It is set that most essential for identification of hybridness of tomato in vitro are signs related to the phenotypical classes of mutants A, J, C, D, E, F, G. The method of rapid and assured reproduction of test tube plants of remote hybrids is developed, normal plants are got. Possibilities of saving of tomato mutant forms are researched. In vitro-collections of androgenic calluses with complex resistance, sterile and hybrid forms of tomato were created. A copyright certificate is got on the culture of tomato Lapochka with the mutant genes of sp, d, br, u. Keywords: tomato, Lycopersicon esculentum, embryonic plasma, remote hybridization, androgenesis, culture of isolated anthers, in vitro-culture, morphogenesis, introgressive breeding. Product Description popup.authors Мірошніченко Віра Петрівна popup.nrat_date 2020-04-02 Close