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Information × Registration Number 0211U002646, 0106U003670 , R & D reports Title To work out a method for a long - term IN VITRO storage of ALLIUM SATIVUM L. collection and ways of restoring "old seeds" of vegetable plants with the help of biotechnological methods popup.stage_title Head Ivchenko Tetiana Volodumurivna, Registration Date 17-02-2011 Organization Institute of vegetable and melons Ukrainian Academy of Agrarian Sciences popup.description2 Object of investigation: genetic collection of garlic plants of a long day. The aim of investigations: to elaborate on the basis of modern methods of biotechnology criteria for creation and long keeping in vitro garlic collections without changing of their genetic constitution. It has been proved experimentally the effectiveness of the use of biotechnological methods for creation and maintenance of garlic collections in living condition. The method of long-term storage of sanitated meristematic garlic material in the conditions of cryogen temperatures (patent №22540) has been worked out and patented, allowing to preserve garlic collection samples (apical meristems) without transplantation up to 20yars, that is more economic from the point of view of using material and power expenses. The method of middle-long storage of garlic collections in conditions in vitro has been worked out. The cultivation of tube plants without transplantation at t° 24 °C in the medium. MS with the high saccharose concentration (up to 120 g/l) essentially slowed down the growth and ensured getting of 84,9 ±4,7 % of plants, possessing vital capacity after 12 months of storage. The stability of morphological indices, which the plants R-1 have, cultivated according to the elaborated methodology, with the initial clones, multiplied after standard technology, has been established. The active collection of garlic in vitro with the use of method of middle-term storage, containing of 41 variety samples of collection NCGRPU was created. The sphere of use: selection and seed growing. Key words: in vitro collection criocoservation, storage, microclonal reproduction, meristem, deposition, regeneration. Product Description popup.authors Івченко Тетяна Володимирівна Віценя Тамара Іванівна popup.nrat_date 2020-04-02 Close