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Information × Registration Number 0211U004244, 0109U001563 , R & D reports Title The investigation of ionizing radiation modificational effect on chromosome stability in somatic cells of a human by means of testing mutagenic exposure of bleomycin popup.stage_title Head Pilinskaya Maria, Доктор медичних наук Registration Date 02-02-2011 Organization Research Centre for Radiation Medicine popup.description2 Object of study: human blood lymphocytes. Purpose: to investigate chromosomal instability in somatic cells of cancer patients who deny conscious contact with mutagenic factors, using the testing mutagenic exposure by bleomycin. Methods: cytogenetics, statistics. Results: With the help of modifying by us "G2-bleomycin sensitivity assay" hidden chromosome instability in patients with lung cancer who deny conscious contact with mutagenic factors had been evaluated. It had been shown that the addition to the background mean frequency of chromosome aberrations (above spontaneous cytogenetic effect) in the group of cancer patients was 44.6 and 53.3 per 100 metaphases (at concentrations of bleomycin 0.05 and 5.00 mcg/ml, respectively), which differed significantly (p<0.001) from that in group of apparently healthy individuals (9.14 and 14.31 per 100 metaphases, respectively). The broad intra-individual variations of induced cytogenetic effect in patients with lung cancer (8.20 - 109.25; 23.20 - 99.58 chromosomal aberrations per 100 metaphases at concentrations of bleomycin 0.05 and 5.00 mcg/ml, respectively) and the absence of positive correlation between background and induced frequencies of chromosomal damages had been established. In the group of cancer patients 53.3% of persons with hidden chromosome instability had been detected, which significantly (p<0.01) differed from that (33.3%) in the group of apparently healthy individuals. Product Description popup.authors Єремєєва Марія Миколаївна Дибська Олена Борисівна Дибський Сергій Сергійович Степанова Людмила Сергіївна popup.nrat_date 2020-04-02 Close