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Information × Registration Number 0211U004471, 0109U004086 , R & D reports Title Mechanisms of influence of physical factors, nanoparticles and biologically active substances an macromolecules and cells popup.stage_title Head Maleev Vladimir, Gatash Sergej, Registration Date 08-02-2011 Organization Kharkov National University named after V.N. Karazin popup.description2 Project report (final): 120 pages, 36 figures, 8 tables, 204 references. Subject of research: blood serum and plasma, platelets, follicular fluid, 5,5',6,6'-tetrachloro-1,1,3,3'-tetraethylbenzimidazolylcarbocyanine iodide (JC-1), ultradisperse diamonds, meso-5,10,15,20-tetra(4-methyl-N-pyridyl) porphine (TMPyP4), poly(A), poly(U), poly(dA), poly(dA)-poly(dT), etidium bromide, proflavin, propidium iodide, DNA. The aims of this work are to investigate the physical mechanisms of interaction of nanoparticles and biological active substances with biomacromolecules and cells under water-ionic conditions. Methods: photoelectrocolorimetry, UHF-dielectrometry, absorption and fluorescent spectroscopy, static light scattering, gel chromatography. Effect of the biological active substance (BAS) quercitin on blood components on experimental metabolic syndrome model of rats was studied. It was established that the deficit of estrogens leads to rising of speed of ADP-induced platelet aggregation and to decreasing of dielectric permittivity (e') of blood serum and plasma, which is probably a result of accumulating of lipid peroxidation products in blood. The effects of a follicle size, biochemical characteristics and freezing on water state in follicular fluid (FF) were studied. A dependence of FF component hydration on a follicle diameter was revealed. Dielectric and electric properties of the colloidal solutions of JC-1 nanoparticles in the presence and absence of cetyl pyridine bromide (CPB) were studied. It was determined, that CPB leads to decreasing of the conductivity values of nanoparticle solutions. The state of water in suspensions of ultradisperse diamonds (UDD) and the influence of UDD on erythrocytes upon irradiation were studied. Two types of structured water, i.e. strong bound with the surface of UDD hydration water (thickness of the layer is 1.8-2 nm) and volume water characterized by reduced values of fd are present in water suspensions. The mechanisms of interaction of synthetic cationic porphyrin meso-5,10,15,20-tetra(4-methyl-N-pyridyl) porphine (TMPyP4) with single stranded polyribonucleotide poly(A) and poly(U) was established. The main types of binding are determined: quasiintercalation, external electrostatic monomer and stacking-associative-binding and the conditions to their appearance. Effects of Ni2+ and Cd2+ ions on thermally induced conformational transitions in the poly(dA)•poly(dT) polynucleotide duplex and poly(dA)•2poly(dT) triplex under near physiological ionic conditions were studied. It was determined that unlike Ni2+, Cd2+ induces formation of very stable aggregates which did not disintegrate even upon cooling up to room temperature. The dielectric properties of water solutions of some BAS (ethidium bromide, proflavine, propidium iodide) and complexes of DNA with these substances were investigated. It was shown that the complexation is accompanied by changes in the hydration shell of DNA. Results of the research were introduced to the method of studying of water state in UDD suspensions and could be used in receiving of new radio protectors at Institute of medical radiology of the National Academy of Medical Science of Ukraine, in developing of diagnostic and medical treatment methods at Institute for problems of endocrine pathology of the National Academy of Science of Ukraine, in receiving the stable colloidal solutions of nanoparticles at Institute of scintillation materials of the National Academy of Science of Ukraine. UHF-DIELECTROMETRY, PERMITTIVITY, SERUM, PLASMA, PLATELETS, PLATELET AGGREGATION, INSULINRESISTANCE, QUERCITIN, FOLLICULAR FLUID, FREEZING, ULTRADISPERSE DIAMONDS, ERYTHROCYTE, HYDRATION, STRUCTURED WATER, TMPyP4, POLY(A), POLY(U), Ni2+, Cd2+, POLY(dA), POLY(dA)-POLY(dT), AGGREGATION COMPKEXFORMATION, ETHIDIUM BROMIDE, PROFLAVINE, PROPIDIUM IODIDE, DNA. Conditions of reception of the report: under the contract. 61077, Kharkov, Svobody sq., 4, V.N. Karazin Kharkov National University. Product Description popup.authors Адельянов О. Гордієнко Є. Горобченко О. Жигалова Н. Ніколов О. Песіна Д. Стенкова Т. popup.nrat_date 2020-04-02 Close