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Information × Registration Number 0211U004480, 0106U006844 , R & D reports Title Develop technology to produce, selection and assessment of recoveries, high performance and high-quality source material potato, resistant to biotic and abiotic factors on the basis of methods of biotechnology popup.stage_title Head Oliynyk T.M., Registration Date 03-02-2011 Organization Institute for potato Research popup.description2 The research on genetic engineering optimized cultivation conditions agrobacterial strains. Allocated in 1928 transformed lines with increased economic indexes for securities: yields up to 67% starch content to 3,6% of dry matter to 4.3% crude protein to 0,4%. Our results on recovery of seed potatoes by meristem apex were 142 asymptomatic lines by thermotherapy - 232, by chemotherapy - 314 lines 48 sortozrazkiv. Research on defining variability healed by chemotherapy lines was made using RAPD-PCR. The research we determined the optimal concentration range of reagents and reaction mixture conditions for RAPD-PCR, in which such changes affect the minimum of results. Worked out the conditions of PCR analysis for detection of genetic structures that contain genes nptII and 35Spromotoru. The research on clustering R.solanacearum strains using RAPD-analysis, the genetic relationship between strains belonging to one biovaru. For the first time R. solanacearum, described the loss of O-polysaccharide, with increasing temperature environment. Also for items. 749 shows cleavage 3 hidroksymirystynovoyi acid from lipid A, which may be associated with gene expression PagL. Product Description popup.authors Белошицька Н.Й. Грицай Р.В. Сідакова О.В. Слободян К.А. Слободян С.О Таращенко Н.І. Шевченко О.О. popup.nrat_date 2020-04-02 Close