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Information × Registration Number 0211U004481, 0106U006846 , R & D reports Title Receipt and analysis of transgenic plant species Solanum tuberosum, resistant to diseases by using Agrobacterium-mediated strains popup.stage_title Head Oliynyk T.M., Registration Date 03-02-2011 Organization Institute for potato Research popup.description2 The studies are optimized culture conditions agrobacterial strains. Noted that the rapid growth of bacteria occurs in media YTG and AB, at an optimum period of growth 72 h, temperature 26 ° - 28 ° C. To maintain strains of Agrobacterium tumefaciens is expedient for a month at 4 ° C. As a result of transformation of explants of stems, and discs microtubers of potato, the strain with the gene of defensin, Otselektirovan at km-stable environment transformed line varieties: Puzzle - 11 lines, Dara - 23 lines, using the coat protein gene of the virus X: Holiday - 23 lines, Fun - 10 lines, Stork - 14 lines , Palette - 7 lines, Slavyanka - 42 lines, Levada - 11 lines, Legionnaire - 23 lines. When tested for resistance to Alternaria vidileno 76,4% of transformed lines, varieties Slavyanka, Festive, Fun, Stork, Palette, Riddle, Dara with high grade resistance (compared with the control), which is 8-9 points depending on the variety. Highlighted 20liny with elevated businesslike valuable indicators for: yield up to 36% starch content up to 1,7% of dry matter to 3.2% of crude up 0.3%, which was tested at various stages of the selection process in the department of plant breeding. According to the research identified two transformed lines Sk5 (Svitanok Kiev) and Sl4 (Slavonian) are on a range of economically important parameters passed into a hotbed of competitive - Environmental testing of breeding. Product Description popup.authors Белошицька Н.Й. Грицай Р.В. Слободян С.О. Таращенко Н.І. popup.nrat_date 2020-04-02 Close