Search academic texts

Information × Registration Number 0211U004482, 0106U006847 , R & D reports Title Improving methods for the process of accelerated reproduction of the original seed potato culture in vitro. popup.stage_title Head Oliynyk T.M., Registration Date 03-02-2011 Organization Institute for potato Research popup.description2 As a result, studies on improvement of seed potatoes by the apical meristem, in combination with thermo-and chemotherapy received 332 virus-free line 13 accessions. Established stimulatory effect virusingibuyuchih substances on plant regeneration. Efficiency of plant regeneration in Class II Borodyansky pink rose by 16,3%, grade Lugovskaya -23.5% grades Dnepryanka Yavor, and Lileya -26-34%, varieties glaze, Dawn of Kiev and Kalinovskaya - 14 - 42%. Established a stimulating effect on the regeneration of meristems using Amiksina. Regeneration takes place in one passage (1-1,5 months) faster compared with the control (meristem). High regeneration capacity from 40 to 94% observed in class Levada. Highest regenerative ability (80%) was achieved by grade Riddles amiksin medium with a concentration of 0.1% and 75% in the medium with ribonuclease. Using RAPD-PCR analizuvstanovleno presence of polymorphism in lines of class Levada redeveloped by chemotherapy. Established lines of variability in the field of productivity within the reduction to 10%, an increase of 30-40%. The high variability of the lines for yield and its components at the next reproduction can be a source of biological contamination of potato varieties. According to the results of diagnosis allocated to a pure line varieties Dnepryanka and Lilia with high resistance to disease, which after checking on the performance can be recorded in the bank redeveloped plants in vitro. The proposed method of estimating the redeveloped accessions, which at the genetic level can analyze samples for variability for the selection of a line identical with the source material in the early stages of recovery. Product Description popup.authors Грицай Р.В. Слободян К.А. Слободян С.О Таращенко Н.І. Шевченко О.О. popup.nrat_date 2020-04-02 Close