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Information × Registration Number 0211U004789, 0109U005309 , R & D reports Title Regulation of the adaptor protein Ruk/CIN85 expression by hypoxia popup.stage_title Head Samoylenko Anatoliy Anatoliyevych, Registration Date 28-02-2011 Organization A.V.Palladin Institute of Biochemistry of National Academy of Sciences popup.description2 Study the mechanisms of action of individual proteins in cellular reply on hypoxia is one of the actual problems in the fields of biochemistry and molecular biology. It was shown that overexpression of adaptor protein Ruk/CIN85 lead to increase of HIF-1? intracellular content and its ability to stimulate the luciferase gene reporter activity with HRE elements of erythropoietin gene promoter. Overexpression of Ruk/CIN85 is also accompanied by increase of intracellular content of PAI-1 protein under normoxia and more substantially under hypoxia. The stimulation of PAI-1 expression under Ruk/CIN85 overexpression depends on the presence of HRE site in PAI-1 promotor. Both increase of HIF-1? content and enhancement of PAI-1 expression do not observe after suppression of high Ruk/CIN85 expression level using RNA-interference approach. It was demonstrated that Ruk/CIN85 expression did not change under hypoxia but increased under the action of insulin and IGF-1 Product Description popup.authors . Дробот Людмила Борисівна Козлова Ніна Володимирівна Марченко Світлана Миколаївна. popup.nrat_date 2020-04-02 Close