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Information × Registration Number 0211U005447, 0110U004405 , R & D reports Title To improve the methods for the creation of rape haploids in anther culture in vitro popup.stage_title Head Bilyns'ka Olena Volodymyrivna, Кандидат біологічних наук Registration Date 24-11-2011 Organization Plant Production Institute nd. a. V. Ya. Yuriev of NAAS popup.description2 The effective method for initiation of a sterile winter rape anther culture in vitro based on the use as a mean of sterilization the commercial preparation "Domestos" has been developed. A comparative study on the efficiency of the 5 nutrient media possessing different macro-, microelement salt, growth regulator composition and sugar component was carried out. In has been revealed that a mineral base of the media had not a sufficient effect on a process of morphogenic structure induction. The largest amount of morphogenic anthers - 41.09 % - was obtained on the medium NMSмод. 2 with a relatively high content of 2,4-D (2 mg/l), but androgenic structures initiated on this media were performed by callus with a low regenerative potential. Sufficient improvement of the embryoid yield was achieved on the medium MSdb possessing 2,4-D and BAP composition Product Description popup.authors popup.nrat_date 2020-04-02 Close