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Information × Registration Number 0211U005493, 0111U006759 , R & D reports Title Studing of biodiversity and structure of microbic communities of podsolic soils. popup.stage_title Head Patyka Nikolai Vladimirovych, Доктор сільськогосподарських наук Registration Date 20-12-2011 Organization National university of Life and Environmental Sciences of Ukraine popup.description2 Object of research - soil microflora, which is formed at agricultural land use in the basic growth phases of agricultural plants, ways of their cultivation, definition of process elements forming production crops.The research objective - research work is directed on the decision of a fundamental problem of preservation of soil microbic complex biodiversity, increase of soil fertility and stability of agroecosystems, studying of biodiversity and structure of microbic complex of the podsolic soil, that formed in the cultivation of crops. To create a model of the formation of microbial complex in a crop rotation link (a clover, flax, a winter rye), to develop methodical approaches of microbic processes management which provide increase of agroecosystems stability. Traditional research methods by seeding the soil suspension on elective nutrient media and modern molecular genetic methods of the analysis of complex microbial structures such as restriction fragment length polymorphism (tRFLP) (MG LaMontagne, 2003), polimerase chain reaction in real time (PCR REAL -TIME) were used for estamination of soil microflora biodiversity (Heid CA, 1996). It was conducted identification and estimated phylogenetic diversity of soil prokaryotic complex based on the received values of genetic diversity. It was used environmental indices of Simpson dominance and Shannon diversity and so on at the structure analysis of prokariotic communities in soil (Odum, 1976). Combining classical and molecular genetic methods of soil microflora enlarged knowledge of genetic resources and microbial complex structure which is formed at long-term agricultural land use that is very important to understand this phenomenon as "soil fatigue" and, finally, to create sustainable and highly productive agroecosystems. Research work with using traditional and modern molecular genetic methods of analysis of the structure and biodiversity of soil microbial complex in conjunction with the analysis of agro-chemical parameters in long-term field experiments conducted for the first time. The sphere of using - agriculture, microbiology, soil science, ecology, molecular biology. Product Description popup.authors Гончар Олена Миколаївна Демченко Вікторія Олегівна Демченко Оксана Михайлівна Демчук Тетяна Леонідівна Канарський Євген Русланович Колодяжний Олександр Юрійович Кошевський Іван Ілліч Ліханов Артур Федорович Лобов Олександр Валентинович Мосійчук Наталія Олександрівна Москалевська Юлія Петрівна Мошковська Людмила Трохимівна Мошковський Микола Сільвестрович Патика Микола Володимирович Патика Тетяна Іванівна popup.nrat_date 2020-04-02 Close