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Information × Registration Number 0211U005949, 0106U005434 , R & D reports Title Research of structural-functional interaction of baculovirusis with cells of eucariotic. popup.stage_title Head Strokovskaya Lyudmila Ivanovna, Solomko Olexsandr Petrovich, Registration Date 20-12-2011 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 In the course of project execution recombinant baculoviruses with the genes of cytokines, which expression is regulated by viral and nonviral mammalian promotes, were constructed; optimal conditions for transduction of different cell types were determined; transduction efficiecy of differing in origin tumor cells by baculovirus has been investigated and it was found out that tumor cells of different origin are characterized by a sufficiently wide range of sensivity to baculoviruses; efficiency of expression of cytokines: mouse interferon -beta and interleukin 2 and 12 within baculovirus vectors in permissive and nonpermissive cells was determined; high efficiency of transduced fetal mouse fibroblasts application as the cellular vectors in in vitro and in vivo mouse model was shown; changes in expression of several interferon-stimulation and epitelian to mesenchymal transistion engaged genes in tumor cells upon the interaction with interferon-beta producing vector cells were investigated. Product Description popup.authors Анопрієнко О.В. Бобровська М.Т. Вагіна І.М. Зінченко В.А. Кіхно І.М. Козлов Е.А. Морозова Л.М. Чащина Л.І. Ширина Т.В. popup.nrat_date 2020-04-02 Close