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Information × Registration Number 0211U006297, 0110U002250 , R & D reports Title Development of main criterion for application of silica sorbents with biomimmetic dye-ligands for receiving diagnostic and terapeutic protein preparation popup.stage_title Head Danysh Taras Vasylovych, Registration Date 09-03-2011 Organization LVIV RESEARCH INSTITUTE OF BLOOD PATHOLOGY AND TRANSFUSIONAL MEDICINE popup.description2 The objects of research are fractions I, II + III and III plasma Cohn fraction of silica sorbents with biomimetic dye-ligands, proteins of blood plasma, prothrombin complex. Research objective: to create sets of biospecsfic sorbents based on macroporous silica gel and ligand - active triazynе dyes, to justify conditions of their use for rapid analysis and purification of proteins with diverse biological material. While the research methods used in chemical synthesis, alcohol, salt and PEG-fractionation, affinity and gel-penetrating chromatography, centrifugation, PAAG-electropho- resis and other methods of analysis of drugs.The following scientific and practical results: 4 new synthesized sorbents based on silica matrix Diasorb-diol containing ligands as active triazyne and vinilsulfon dyes; their physical and chemical properties that characterize their high resistance and stability during the chromatographic studies of protein solutions. Application: blood transfusion stations, chemical and pharmaceutical companies, research organizations. Equipment: prepara- tive centrifuges, chromatographic columns with carriers, spectrophotometer, instrument for electrophoresis, preparative chromatograph and others.Degree of implementation: the results obtained are the subject of laboratory regulations of use of silica biomimetic human proteomic analysis of sorbents, presentations at international scientific forums, publications in scientific literature. Efficiency: creating sets biomimetic sorbents with the predicted conditions for their use to quickly analyze the protein composition of biological fluids.The combination of bio-targeting chromatography methods of enzymatic hydrolysis, two-dimensional electrophoresis and determination of specific activity and facilitate the study of metabolic proteomix. Bioimimetic sorbents can be used in technology selection and purification of protein factors, including recombinant. Application of silica sorbents can be easily combined with methods of inactivation hemotransmission infections while receiving drugs from plasma. Product Description popup.authors Вороняк М.І. Вус М.М. Даниш Т.В. Дульцева Н.А. Дяків Х.І. Мадич С.Є. Орлова Л.В. popup.nrat_date 2020-04-02 Close