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Information × Registration Number 0211U006827, 0108U001295 , R & D reports Title Biological characteristics of diphtheria, whooping-cough and tuberculosis pathogen antigens, that were isolated with the help of physicochemical methods. popup.stage_title Head Babych Evgenij Mihailovich, Registration Date 11-04-2011 Organization The Mechnikov Research Institute of microbiology and immunology at Kharciv popup.description2 Conducted investigations are devoted to development of approaches to improvement of diphtheria and whooping-cough vaccines notably directed at increasing antibacterial immunity as a factor of diphtheria infection localization in organism, development acellular whooping-cough vaccine, obtaining adjuvant preparation from mycobacterium tuberculosis. For obtaining native antigens mentioned pathogens it was used next technological operations. At the first stage of investigations the most perspective regime of ultrasound disintegration microbe cells C. diphtheria and B. рertussis was characterized. Then for obtaining antigen preparation it's used centrifugation, ultrafiltration, ultracentrifugation and preparative gel-chromatography. Obtained samples of C. diphtheria, B. pertussis, M. bovis antigen preparations were characterized for their chemical structure and molecular weight. It's studied the influence of experimental preparations of subcellular C. diphtheriae complexes samples on dynamic of humoral diphtheria immunity and non-specific immune protection (phagocytosis activity) during immunization of laboratory animals. It's studied the influence ultracentrifugation on obtaining of antigenic fractions B. pertussis and their biochemical composition. It's studied toxic, dermonecrotic, immunogenic, adhesive properties of B. pertussis microbe cells antigenic complexes preparations during immunization of laboratory animals. It's obtained perspective for development acellular whooping-cough vaccine non-toxic immunogenic fraction with molecular weight 8,1 кDа. It's studied adjuvant properties M. bovis antigenic preparation to rabbit antitoxic diphtheria immunity generation. According to experiments data it's elaborated the project of analytic-preparative set for native antigens bacterial cells obtaining model which make possible to combine conducted experiments into a single whole process. Product Description popup.authors Єлисеєва Ірина Віталіївна Ісаєнко О.Ю. Білозерський Володимир Іванович Бабич Євгеній Михайлович Ждамарова Ларсиа Анатоліївна Колпак Світлана Анатоліївна Скляр Надія Іванівна popup.nrat_date 2020-04-02 Close