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Information × Registration Number 0211U008681, 0111U007143 , R & D reports Title Impact studying of cultured multipotent mesenchymal stromal cells of various origin on burn and wound healing popup.stage_title Head Ostapchenko Liudmila I., Registration Date 26-08-2011 Organization Taras Shevchenko Kiev university popup.description2 The full-thickness burn was generated with a preheated brass plate application on a mouse skin, a day later the necrectomy was accomplished, then the living dermal equivalent, consisting of polymerized collagen gel and cultured syngeneic MSCs from dermis (1 x 106 cells/ml), was implanted to the post-necrectomy burn wound of animals from experimental group of CBA mice. It was resulted, in vitro MSCs cultures isolated from different tissues of C57BL/6 and CBA mice (dermis, thymus stroma, adipose tissue, bone marrow stroma) were obtained. The tests for osteo- and adipogenesis differentiations of cultured MSCs were also carried out. In the control group of CBA mice without a living dermal equivalent implantation, the non-healing and the wound contraction to the 20th day post-burn was observed up to 32% (100% - 2nd day post-necrectomy). At the same time, the same value in the experimental CBA mice group with a dermal equivalent implantation was, on average, to the 15th day - 16%, and to the 20th day post-burn the resulted wound was completely healed. Product Description popup.authors Галицька С. М. Зубов Д. О. Остапченко Л. І. popup.nrat_date 2020-04-02 Close