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Information × Registration Number 0211U008901, 0106U005435 , R & D reports Title Organization and regulation of ITSN-dependent signal transduction pathways popup.stage_title Head Ryndych Alla Volodymyrivna, Доктор біологічних наук Registration Date 29-11-2011 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 Adaptor proteins of intersectins (ITSN) family are involved in clathrin-mediated endocytosis, mitogenic signaling and actin cytoskeleton rearrangements. We have shown the role of alternative splicing in regulation of the binding properties of ITSN isoforms. It was demonstrated the neuron-specific expression of ITSN1 isoforms containing exon 20. In vitro binding experiments demonstrated that inclusion of exon 20 changes the binding properties of the SH3A domain of ITSN1 towards several endocytic and signalling proteins. The novel shortest isoform of ITSN1 with an alternative C-terminus was identified and characterized. This isoform is only found in mammals and is characterized by specific protein interactions. Moreover, we have identified seven novel protein partners of ITSN1 and ITSN2 and found a complex of ITSN with latent membrane protein 2A of Epstein-Barr virus. Using Xenopus animal model we have demonstrated the role of ITSN2 in the coordinated changes of actin cytoskeleton during early embryonic development. Product Description popup.authors Базан Тамара Адамівна Грязнова Тетяна Анатолівна Дергай Микола Васильович Дергай Олександр Васильович Кропивко Сергій Вікторович Ніколаєнко Олексій Валентинович Новохацька Ольга Василівна Скрипкіна Інесса Яківна Фоєнко Інна Миколаївна Циба Людмила Олексіївна Чаїн Людмила Олександрівна popup.nrat_date 2020-04-02 Close