Search academic texts

Registration Number

0211U009255, 0111U006628 , R & D reports

Title

Immunoproteomic analysis of autoantigenic repertoire of unusually indolent tumors associated with prominent lymphocytic infiltratrion.

popup.stage_title

Head

Filonenko Valeriy Viktorovich, Доктор біологічних наук

Registration Date

30-09-2011

Organization

Institute of Molecular Biology and Genetics of NASU

popup.description2

Object of study: autoantigenic repertoire of medullary breast carcinoma and papillary thyroid carcinoma. Aim of the project - to clone and to obtain purified recombinant proteins of potential autoantigens associated with medullary breast carcinoma and thyroid cancer that were previously identified by SEREX-analysis. To make an immunization of mice with obtained antigens Methods - biochemical, molecular, genetic, genetic engineering. cDNA of potential autoantiges that were identified by SEREX-analysis of antigenic repertoire of medullary breast carcinoma (RAD50, TOP2B, FAM50A, LGal3BP, HMGN2, PARD3, SAP30BP, ANKRD11, SPP1, PDCL, PABCP4, RBPJ, LRRFIP1) were cloned in vectors for expression in bacterial cells (pGEX4T3, pET42b, pET28b). Purified recombinant proteins and fragments of potential autoantigens associated with medullary breast cancer were obtained: С/RAD50, RAD50(36), TOP2B, FAM50A, LGal3BP, HMGN2, PARD3, SAP30BP, ANKRD11, SPP1, PDCL, PABCP4, RBPJ, LRRFIP1, NY-BR-1, NY-BR-62, NY-CO-58. Also purified recombinant protein NaPi2b potentially associated with thyroid cancer was obtained. Mice were immunized with recombinant proteins С/RAD50, FAM50A, LGal3BP, PABCP4, RBPJ, LRRFIP1 and NaPi2b for obtaining of polyclonal antibodies. Keywords: autoantigens, recombinant proteins, cloning, protein expression, immunization.

Product Description

popup.authors

Кіямова Р.Г.
Костянець О.І.
Савінська Л.О.
Хоруженко А.І.

popup.nrat_date

2020-04-02