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Information × Registration Number 0211U009255, 0111U006628 , R & D reports Title Immunoproteomic analysis of autoantigenic repertoire of unusually indolent tumors associated with prominent lymphocytic infiltratrion. popup.stage_title Head Filonenko Valeriy Viktorovich, Доктор біологічних наук Registration Date 30-09-2011 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 Object of study: autoantigenic repertoire of medullary breast carcinoma and papillary thyroid carcinoma. Aim of the project - to clone and to obtain purified recombinant proteins of potential autoantigens associated with medullary breast carcinoma and thyroid cancer that were previously identified by SEREX-analysis. To make an immunization of mice with obtained antigens Methods - biochemical, molecular, genetic, genetic engineering. cDNA of potential autoantiges that were identified by SEREX-analysis of antigenic repertoire of medullary breast carcinoma (RAD50, TOP2B, FAM50A, LGal3BP, HMGN2, PARD3, SAP30BP, ANKRD11, SPP1, PDCL, PABCP4, RBPJ, LRRFIP1) were cloned in vectors for expression in bacterial cells (pGEX4T3, pET42b, pET28b). Purified recombinant proteins and fragments of potential autoantigens associated with medullary breast cancer were obtained: С/RAD50, RAD50(36), TOP2B, FAM50A, LGal3BP, HMGN2, PARD3, SAP30BP, ANKRD11, SPP1, PDCL, PABCP4, RBPJ, LRRFIP1, NY-BR-1, NY-BR-62, NY-CO-58. Also purified recombinant protein NaPi2b potentially associated with thyroid cancer was obtained. Mice were immunized with recombinant proteins С/RAD50, FAM50A, LGal3BP, PABCP4, RBPJ, LRRFIP1 and NaPi2b for obtaining of polyclonal antibodies. Keywords: autoantigens, recombinant proteins, cloning, protein expression, immunization. Product Description popup.authors Кіямова Р.Г. Костянець О.І. Савінська Л.О. Хоруженко А.І. popup.nrat_date 2020-04-02 Close