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Information × Registration Number 0211U013052, 0110U006191 , R & D reports Title Use of effective oligoelectrolyte systems for delivery of genes into cells for genetic transformation of plants popup.stage_title Head Stoika Rostyslav, Registration Date 15-12-2011 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 It was modeled potential delivery system of nucleic acids into plant cells, as well as their test synthesis, separation and purification. It was optimized protocol of purification and separation of nanoscale polymer systems for delivery of nucleic acids. It was synthesised polymers based on poly-DMAEM. It was investigated DNA-binding properties of polymers, the optimum parameters of the solution ionic strength, pH, time and temperature regimes for optimal binding with plasmid DNA. It was optimized a protocol of foreign DNA delivery based on the using of poly-DMAEM carriers into plant's protoplasts, in particular, Nicotiana tabacum. It was studied the effect of duration of treatment (time of cultivation of carrier with plasmid DNA), the concentration of polymeric poly-DMAEM carrier and concentration of DNA on the survival of the protoplasts. It was elucidated the cytotoxicity of the developed polymeric carriers during regeneration of protoplasts in culture in vitro. Product Description popup.authors Філяк Євген Зеновійович popup.nrat_date 2020-04-02 Close