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Information × Registration Number 0211U013087, 0111U005988 , R & D reports Title Macromolecules and their complexes in realization of genetic information popup.stage_title Head El'skaya Anna Velentynivna, Доктор біологічних наук Registration Date 16-12-2011 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 In the first phase of research ше was planned to make structural and functional analysis of macromolecular complexes involved in the catalysis of DNA polymerase, editing aatRNK-synthetases, translation, elongation, isoforms of S6K, and genome methylation processes reoxygenation. Thus, using modern quantum-chemical modeling methods, physical and chemical nature of spontaneous point errors of DNA replication was investigated. At the first timeit was received the strong arguments to support of tautomeric hypothesis of point mutations. A comparative study of the edition process by prokaryotic leytsyl-tRNA synthetase from Thermus thermophilus (LeyRSTT) of 7 nonhomological amino acids that are similar in structure to leucine. Significant difference was detected between compact spatial structures highly conservative eEF1A isoforms which may be considered as molecular basis for providing different range of the interactions specific for each isoform with specific partner proteins in cancer cells, where A1 and A2 probably perform their non-canonical functions. Using bioinformation analysis a number of hypothetical isoforms of ribosomal protein kinase S6 (S6K1 / 2) and mTOR kinase were identified. For functional analysis of a Lizophorm, S6K2/?22, a stable cell line is created corresponding to a model which demonstrated its potential oncogenic properties. On the other hand, with the help of NotI-mikromatryts genetic / epigenetic changes in 115 and 137 loci/genes for tumors of the kidneys and intestine, respectively, were identified. By real time PCR it was also set the quantitative changes in 4 miRNAs in cultured cardiomyocytes modeling reoxygenation and anoxia of different duration. Thus, as a result of Stage 1 of the research project "Macromolecules and their complexes in the realization of genetic information" heterogeneous structural properties of macromolecular complexes and their components were studied related to the performance of their biological functions, and the prospects of further research in this direction were determined. Product Description popup.authors Говорун Дмитро Миколайович Кавсан Вадим Мусійович Негруцький Б.С. Риндич Алла Володимирівна Філоненко В.В. popup.nrat_date 2020-04-02 Close