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Registration Number

0211U013167, 0110U005689 , R & D reports

Title

Biological activity and safety of nanomaterials that could be used in development of vector systems of antitumor drugs

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Head

Chekhun Vasyl Fedorovych,

Registration Date

18-01-2012

Organization

R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology

Description

We studied cytotoxic and genotoxic effect of nanomaterials of different origin: nanoferromagnetics and magnetic fluid in concentrations 3 and 6 mg/kg (weight) and colloid gold in concentrations 30 and 60 mg/kg (weight) on Ehrlich ascetic carcinoma cells after 24 and 48 hours. We showed that the degree of cytomorphological and ultrastructural changes, caused by nanoferromagnetic, correlated with its doses and duration of its imact. Thus we suggested that it waspossible to detect degree of cytotoxic effect caused by the studied nanomaterial using methods listed above. We found that nanoferromagnetic caused higher cytotoxic effect in tumor cells than magnetic fluid and colloid gold did. This resulted in vacuolar-hydropic dystrophy, including mitochondrial vacuolarisation, development of different degrees of blebbeing in cytoplasm and some nuclear perturbations (early chromosome condensation, chromatin perturbations, fragmentation of nuclei, formation of protrusions and micronuclei), which were markers of genotoxicity. Our previous in vivo results about cytotoxic effects of nanoferromagnetics on the viscera of mice bearing Ehrilch ascetic carcinoma and in vitro data obtained during this stage gave no reasons to suggest about total safety of tested nanoferromagnetic and pointed on the need of future research. Magnetic fluid and colloid gold did not show significant damage on Echrlich ascetic carcinoma cells based on the results of cytomorphological and ultrastructural features what pointed on their safety in development of vector systems for antitumor drugs. We showed that light and electron microscopy as well as micronuclear test are objective methods in studies of cytotoxic and genotoxic action of nanomaterials on biological objects.

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Бурлака Анатолій Павлович
Лук'янова Наталія Юріївна
Мельник Наталія Миколаївна
Налєскіна Леся Анатоліївна
Русецька Наталія Віталіївна
Тодор Ігор Миколайович
Трегубова Надія Андріївна
Трембач Людмила Валентинівна
Хаєцький Ігор Костянтинович
Чехун Василь Федорович
Юрченко Ольга Василівна