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Information × Registration Number 0211U013185, 0111U005847 , R & D reports Title Assessment of morphological and functional characteristics of hematopoetic stem cells and their nearest progeny in cell culture in vitro popup.stage_title Head Bilko Nadiia Mykhailivna, Registration Date 30-12-2011 Organization National University of "Kyiv-Mohyla Academy" popup.description2 Object of investigation: conditions for the long-term culture of cord blood hematopoietic progenitor cells in culture in vitro. Aim of the investigations: functional assessment of hematopoietic cells population, which are capable to restore hematopoiesis in case of auto- and allotransplantation. Methods: hematopoietic cell culture in vitro, clonogenic analysis in semisolid agar, morphological methods, variation statistics. Theoretical and practical results: analysis of hematopoietic progenitors colony-forming efficiency in cell culture in vitro was performed, as well as morpho-functional characteristics of the closest progeny of stem cells. The most perspective source of stem cells was cord blood. The most appropriate for using in culture was the combination of interleukin-3, interleukin-6 and granulocyte-macrophage factor, which provided high level of colony-forming activity of cord blood cells in vitro. Novelty: possibility of objective evaluation of functional activity of stem and progenitor cells as the potential transplant. Efficacy of implementation: obtained scientific data can be used as a background for development of the expansion protocols for hematopoietic stem cells in vitro culture to obtain sufficient quantity for further uses in experimental and clinical research. Area of implementation: haematology, biotechnology. Product Description popup.authors Білько Денис Іванович Білько Надія Михайлівна Бараш Олексій Олександрович Борбуляк Ірина Зіновіївна Василовська Світлана Володимирівна popup.nrat_date 2020-04-02 Close