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Information × Registration Number 0212U000431, 0107U000443 , R & D reports Title Development of the Scientific Bases of the Biotechnology of Irrehlaccable Amino Adids. Development of the genefically modified (GM) Lysinee producer Brevibacterium sp. popup.stage_title Head Shulga Sergiy Michaylovich, Кандидат фізико-математичних наук Registration Date 10-02-2012 Organization Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine popup.description2 Based on the analysis of metabolic pathways of lysine biosynthesis, enzymes and genes, it was shown that the major genes are pys, lys C, dapA, mgo, lys A, represented by their sequence. Performed clonal analysis for five producers from "Collections" SI IFBG NAS of Ukraine, which produced 6-12 g/dm3 of lysine. It was established that the investigated clones remained auxotrophic for leucine and become auxotrophic for threonine, methionine, tryptophan, and homoserine. Performed mutagenesis using NTG and UV, new clones producing strains was obtain. The new M-strains were selected from regulatory and analog rezistent auxotrophic as genetic markers. Vector systems and the schemes of transformation were chosen for gene pyc, lys C, dapA. The optimum conditions for the cultivation of new M-strains were selected. The biosynthetic activity of these M-strains for lysine (50-60 g/dm3) and conversion rate of sugar (45%) under conditions optimized were defined on laboratory equipments. Product Description popup.authors Андріяш Г.С. Бейко Н.Є. Заболотна Г.М. Тігунова О.О. Ткаченко А.Ф. Хоменко А.І. Шульга С.М. popup.nrat_date 2020-04-02 Close