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Information × Registration Number 0212U001099, 0107U000086 , R & D reports Title Studying and using genetic potential of microalgae from Ukraine for creating environmentally friendly biotechnologies popup.stage_title Head Zolotareva Elena Kostantinovna, Registration Date 16-01-2012 Organization M.G. Kholodny Institute of Botany NASU popup.description2 The objects of researches were algologically clean cultures of cyanobacteria from the collection of Institute of botany of NAS of Ukraine IBASU - B: Nostoc linckia, Spirulina platensis and also microalgae Nannochloropsis limnetica, Botryococcus braunii, Chlamydomonas reinhardtii. Aim of the work: study of dynamics of lipid and fatty acid composition of microalgae of the collection IBASU - B under the influence of external factors with the purpose of production of lipids and polyunsaturated fatty acids. Research methods: algological, biochemical, physicochemical, spectral. The results: Quantitative relations between the separate classes of polar lipids and their content in cells differ considerably in Spirulina platensis and Nostoc linckia. Spirulina platensis is characterized by higher content of monogalactosyldiacylglycerol and phosphatidylglycerol and somewhat lower relative parts of glycolipids in the total quantity of polar lipids. Cultivation of Cyanophyta in the presence of exogenous organic substances results in the redistribution of the ratio between glycol- and phospholipids in favour of phosphatidylglycerol at the expense of monogalactosyldiacylglycerol, and also, in most cases, in the increase in relative content of oleate in lipids along with the decrease in that of palmitoleate. Similarity of the effects observed in Cyanophyta under the influence of glucose and its analogues testifies to the performance of the signalling role by glucose in the cells of Cyanophyta. Addition of methanol improves the functional state of photosynthetic machinery and enhances the rate of electron transport between the photosystems. Results showed that methanol stimulated the processes of photosynthetic transformation of light energy by the cells of C. reinhardtii. Methanol causes the quantitative changes in the ultrastructure of C. reinhardtii also, diminishing the sizes of cells and partial volume of chloroplast, and increasing the partial volumes of vacuoles, mitochondria and plastoglobules. Catalase localized in mitochondria is activated by methanol that allows assuming its participation in oxidation and metabolization of this solvent. Fatty acid composition of microalga Botryococcus braunii and Nannochloropsis limnetica can be modified by the variation of composition of nutrient media. Product Description popup.authors Білявська Нінель Олександрівна Михайленко Наталья Феліксівна Онойко Олена Борисівна Подорванов Володимир Вікторович Степанов Сергій Степанович Шнюкова Єлізавета Іллівна popup.nrat_date 2020-04-02 Close