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Information × Registration Number 0212U001345, 0110U006053 , R & D reports Title Development of bioselective membranes on the base of carbon nanomaterials functionalized by biomolecules for creation of analitical microdevices of new generation. popup.stage_title Head Biloivan Olga Anatoliivna, Korpan Yaroslav Izidorovych, Registration Date 02-02-2012 Organization Institute of Molecular Biology and Genetics popup.description2 Purposely to estimate the reactivity of multi-walled carbon nanotubes (MWCNT) and nanodimonds (ND) was made a study of its low-temperature heat capacity. Reliable standard values of heat capacity, enthalpy, entropy and reduced Gibbs energy were calculated for MWCNT and recommended for practical use for the first time. The analysis of possible impact of surface and intercalated in the crystal structure gas-forming dashes on the nanocrystalline diamond was made. It was demonstrated that the vacuum heat treatment is a promising method of purification of ND from its gas-forming dashes. Method of MWCNT and single-walled carbon nanotubes (SWCNT) functionalization by carboxyl groups and conditions of slurrying of SWCNT and MWCNT samples with different functional groups on their surface were optimized. The optimized biosensor with a MWNCT(NH2) membrane with glucose oxidase was used for glucose determination in wine. Correlation of results of measurements by biosensor method and the method of high-performance liquid chromatography was shown. A series of nanocomposite amperometric biosensors based on immobilized oxidoreductases (glucose oxidase and choline oxidase ) involving carbon nanoparticles (MWCNTs modified by the amino groups and carboxyl groups, SWCNT s and NDs) were developed for glucose and choline detection and their properties were studied. It was shown that modification of gold electrode surface with a layer of carbon nanoparticles before the enzyme immobilization promotes the extension of concentration linear range for substrate determination and the reduction of detection limit in 5-10 times (0,02 mM). Modification of the enzymatic membranes of amperometric biosensor by carbon nanoparticles helps to reduce the enzyme content in the membrane in 5-10 times, increase sensitivity and extend the concentration linear range for substrate determination in 1.5-2.0 times and, in the case of nanotubes, allows to measure substrates at a lower potential (0,2 - 0,5 V). Product Description popup.authors Білоіван О.А. Васильєв О.О. Гарбуз В.В. Дзядевич С.В. Дуда Т.І. Корпан Я.І. Муратов В.Б. Пєшкова В.М. Рогальова Н.С. Солдаткін О.О. Солдаткін О.П. Чабанова М.В. Шкотова Л.В. popup.nrat_date 2020-04-02 Close