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Information × Registration Number 0212U002042, 0109U001322 , R & D reports Title Research of mechanizms of local formation of responce of biological systems and physical principles of new method of medical diagnostic popup.stage_title Head Tovstjak Volodimir Vasilevich; Barannik Evgen Olexandrovich, Registration Date 16-01-2012 Organization Kharkov National University named after V.N. Karazin popup.description2 The aim is to study self-organization of components of biological objects, including nanostructured molecular assemblies, mechanisms of action of various physical factors, the physical laws governing the formation of the local response of biological objects and the study on this basis, the diagnostic capabilities of the new hybrid methods of diagnostics and biosensor systems for medical purposes. The objects of the study are the physical processes and mechanisms of formation of the local response of complex biological systems, depending on their functional status and operation of various physical factors. Proposed and studied physical model for describing their own and forced the fluctuating motion isometric muscle contractions. Ultrasonic Doppler response by inducing muscle contractions is theoretically investigated. It is shown that the spectral characteristics of the local movements of the muscles measured by the Doppler method, associated with a spectrum of perturbations in the muscle fiber as a mechanical system. Spectral characteristics of local isometric contractions of muscle tissue in vivo were studied by Ultrasonic Doppler method. The regularities of the pairing of mechano-chemical processes in contracting skeletal muscle characteristics of the Doppler response of their functional state were established. It is established that the main difference between the amplitude-frequency characteristics of isometric muscle vibration under the action of loads is the amplitude of the fluctuations, while, as the spectral characteristics remain almost unchanged. A model of the mechanochemical coupling processes in contracting skeletal muscle response characteristics of the Doppler and spectral characteristics of the local displacements of skeletal muscle was developed. The algorithms of signal processing for ultrasonic Doppler response diagnostics were developed. The model and hyperthermia response in soft tissues under the influence of powerful wave beams and under the influence of external load was experimentally studied using the laboratory model of the Doppler ultrasonic diagnostic system. As part of the continuum model of the scattering inhomogeneities, expressions correlation functions and spectra of the Doppler response of soft tissues and blood stream lines with the influence of various physical factors was obtained. The influence of the Doppler method and the value of the correlation radius of inhomogeneities and diffusion processes on the spectra generated by stationary flows were investigated. It is shown that increasing the correlation radius of inhomogeneities and the inclusion of their diffusion leads to an additional broadening of the Doppler spectrum. For the vibroelastografy method, which can be used to diagnose cancer in soft tissues, the analytical expressions for the correlation functions and Doppler spectra was obtained. Based on the characteristics of obtained Doppler spectra the possibility of differentiation of soft tissues in amplitude of induced oscillations was shown. The obtained results allow to draw conclusions about the influence of various physical processes and characteristics of biological tissue on the Doppler spectrum and thus increase the effectiveness of medical research. The methods of absorption and fluorescence spectroscopy a number of chromo-and fluorophores to assess their possible use as a reporter molecule biosensor and determination of the spectral parameters of the molecules of reporter protein in the films of lipid mono-and bilayers were screened. The procedures of co-immobilization analytselective and reporter components of biosensors in liquid-crystal membranes the structural and functional properties of immobilized proteins was investigated. The prototypes of optical biosensors were created. The dependence of the spectral response chromo- and fluorophores on the concentration of analytes were elucidated. With the methods of absorption and fluorescence spectroscopy of spectral properties of a number of chromo-and fluorophores in the system glucose + glucose were studied. An evidence of the presence of glucose molecules on the surface binding sites for LF, MCH, FCH, HC, AL, BIS, MBA, MPA, DSP-6, DSP-12 and R8-1350. were obtained. The main role of hydrophobic interactions in stabilizing the complexes of dyes with glucose oxidase was found. The interaction of dyes and fluorescent probes to the apo-glucose oxidase was investigated. It is established that the preparation of apo-GO, isolated from the enzyme-chromogenic mixture, has the greatest affinity for the DSM, DSP-6 and CPD-12, but the intensity of fluorescence of these probes did not change upon binding glucose with apo-GO. We characterize the spectral properties of the mediator dye DCPIP, PMS and MB on the system glucose + glucose in the buffer solution and a suspension of lipid vesicles of different composition. It is shown that the surface immobilization of GO ha FH / CTAB and FH / CL liposomes leads to a decrease in the effective rate constant due to DCPIP reduced efficiency of electron transfer between the dye and enzyme. Biphasic nature of the change in absorption of FMS associated with GS in the presence of lipid membranes was revealed. The feasibility of using gel Toyopearl-60W for the immobilization of the components of biosensors was demonstrated. Product Description popup.authors Баранник Є. Гірник С. Горбенко Г. Житняківська О Кулібаба А. Курилко С. Скресанова І. Товстяк В. Толстолузький Д. Трусова В. Шашель Я. Юдінцев А. popup.nrat_date 2020-04-02 Close