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Information × Registration Number 0212U002177, 0109U001562 , R & D reports Title Investigation of ionizing radiation impact on fertilization potential and physiology-biochemical characteristics of human sperm popup.stage_title Head Andreychenko Sergiy, Registration Date 27-01-2012 Organization Research Centre for Radiation Medicine popup.description2 Object - human sperm. Aim - to study the effect of different doses of external irradiation on physiological and biochemical properties of human sperm and spermatozoid fertilization possibilities; to develop a comprehensive index of fertilization sperm potential assessment and apply it to the general characteristic of the male reproductive function characterizing the inhabitance from radiation polluted Ukrainian regions. Methods - radiobiological, biochemical, microscopic, radioimmunologic, spectrophotometric, flow cytometric and statistical. Human cryopreserved sperm was used in the model experiments to identify radiosensitive parameters: level of free radicals, activity of SOD, hyaluronidase and acid phosphatase, the concentration of cAMP. Irradiation of sperm in vitro in dose range of 1-80 Gy was not critical for the realization of capacitation, hyperactivation, acrosomal reaction and penetration, but sperm irradiated at 100-500 Gy retained the ability to only capacitation and hyperactivation. In randomized cohort studies of donor's sperm quality from different radiation polluted Ukrainian regions was developed a comprehensive evaluation index of human spermatozoid fertilization potential, which revealed correlative dependence on the accumulated radiation dose. The method of long-term cryostorage of human sperm with minimal loss of quality was created Product Description popup.authors Андрейченко Катерина Сергіївна Булавицька Вероніка Михайлівна Ватліцов Денис Володимирович Ватліцова Ольга Станіславівна Клепко Алла Володимирівна Кондратова Юлія Анатоліївна Мотрина Оксана Анатоліївна Пушкаренко Віталій Михайлович Пчеловська Світлана Анатоліївна Чернишов Андрій Вікторович popup.nrat_date 2020-04-02 Close