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Information × Registration Number 0212U002284, 0109U005083 , R & D reports Title Mechanism of the influence of physical factors and biologically active substances on the metabolism and functional properties of connective tissue cells popup.stage_title Head Perskiy E., Registration Date 02-02-2012 Organization Kharkov National University named after V.N. Karazin popup.description2 The influence of mechanical load in the skin of 3-month old rats on proline hydroxylation, hydroxyproline hydration, ?-amino-groups content in the lysine and hydroxylysine, aldehyde groups content in the allysine and hydroxyallysine, the Schiff bases content and the temperature of denaturation in the type I collagen that was synthesized at stretch conditions, the role of some intracellular, membrane and intracellular molecular structures in the chain of transmission of mechanical stress in the connective tissue and the role of the amphiphilic compounds in the resistance of erythrocytes was investigated in vitro. Fluorimetry and IR-spectroscopy has shown that mechanical load in skin decreases the values of proline hydroxylation and hydroxyproline hydration in collagen. The free lysine and hydroxylysine ?-amino-groups in collagen increases, but amount of free aldehyde groups decreases. The Schiff bases content in the collagen supramolecular structures decreases too. Calorimetric measurements of denaturation parameters of the collagen have shown that fibrils formed from synthesized at mechanical stress in skin collagen, have lower temperature and enthalpy energy, as well as lower of intermolecular cross-links - Schiff bases - in contrast with those parameters in the absence of the stress. It proves that cell deformation may influence on protein structure by change of processing level. It is shown, that the signal value of the mechanical stress transmitted to intercellular supramolecular protein-polysaccharide matrix formations, is proportional to the degree of covalent cross-linking. N-glycans of cell surface are the site of the membrane transmission of the signal. It is revealed, that actin complex of the cytoskeleton and that of Са2+/calmodulin - is its separate intracellular sites of the chain of transmission of the mechanical stress signal. It is shown, that the structural conditions of these sites defines the character of feedback in the "cell-intracellular matrix" system. Amphiphilic compounds have been established to significantly increase the resistance of erythrocytes of different mammalian species to hypertonic stress and hypertonic cryohemolysis. The efficiency of the amphiphilic compounds are erythrocyte species-depended Product Description popup.authors Бондаренко В.А. Бондарь І.М. Гоенага М.В. Корнієнко Є.М. Костіна Т.В. Кот Ю.Г. Морозова К.С. Нікітіна Н.А. Пономарьова С.Г. Седлецкая Е.М. Чугай Т.А. popup.nrat_date 2020-04-02 Close