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Information × Registration Number 0212U002355, 0111U008741 , R & D reports Title Light-dependent hydrogen production by microalgae (Chlorophyta) and photosynthetic bacteria (Rhodobacter) popup.stage_title Head Zolotareva Elena Kostantinovna, Registration Date 10-02-2012 Organization M.G. Kholodny Institute of Botany NASU popup.description2 The objects of research were purple bacteria obtained from natural reservoirs of Ukraine, as well as algological pure culture of Chlamydomonas reinhardtii. The aim of the work was isolation, purification, and introduction of the new strains of purple photosynthetic bacteria in the culture, and studying ultrastructural changes of algal cells in the process of hydrogen evolution. Methods: microbiological, biochemical, physico-chemical and spectral. Purple bacteria of the genus Rhodobacter were isolated from 4 natural sources and partially purified for future research. The cells of anaerobic photosynthetic organisms were accumulated for 70 day in the Winogradsky column. Enriched cultures were purified by repeated reseeding on agar medium. Identification of the pink or purple colonies was performed using morphological and biochemical tests, as well as the absorption spectra of cell suspensions and extracts. The formation of H2 according to the equation: CO + H2O > CO2 + H2 was induced very quickly (within 10-30 s) when CO bubbled through a suspension culture. The reaction was relatively insensitive to the presence of oxygen, with the degree of inhibition of the reaction is substantially less in comparison with the processes of the photosynthetic H2 evolution by green algae. Ultrastructural changes in the cells of microalgae C. reinhardtii in response to adding exogenous sources of carbon and induction of hydrogen production were studied by electron microscopy. It was shown the cellular structures varied considerably at transition to oxygen-free cultivation. The area of starch grains firstly increased and then decreased and the decay of granal structure of thylakoids took place. These changes indicate that intracellular starch is used for hydrogen formation. Product Description popup.authors Білявська Нінель Олександрівна Подорванов Володимир Вікторович Поліщук Олександр Васильович Степанов Сергій Степанович Шнюкова Єлізавета Іллівна popup.nrat_date 2020-04-02 Close