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Information × Registration Number 0212U002617, 0110U006268 , R & D reports Title Termo TRP in norm and pathology. popup.stage_title Head Shuba Yaroslav M., Доктор біологічних наук Registration Date 12-01-2012 Organization International center of molecular physiology popup.description2 By means of real-time RT-PCR and immunefluorescent staining combined with confocal microscopy show for the first time the expression of mRNA and protein of the cold/menthol-sensitive cationic channels, TRPM8, in the smooth muscle cells (SMC) from the epididimal and prostatic portions of the rat vas deferens. Expression of TRPM8 mRNK correlated with the expression of mRNK for androgen receptor: orchidectomy of the animals resulted in the enhancement of the expression of both mRNK. TRPM8 protein in the SMC from prostatic portion vas deferens primarily localized outside the plasma membrane, however, in SMC from prostatic portion higher TRPM8 protein targeting specifically to the sarcoplasmic reticulum (SR) and plasma membrane. Non-specific TRPM8 agonist menthol was shown to inhibit voltage- and agonist-evoked contraction of smooth muscle of rat vas deferens. Our date indicate that in the prostatic portion TRPM8 modulates contractility by primarily decreasing the SR Ca2+ stores content, whilst in the epididymal one by both decreasing the SR filling and supporting Ca2+ entry. Castration changes the menthol- and isilin-modiated modulation of the rat vas deferens contractility. Product Description popup.authors Падурару О.М. Філіпов І.Б. popup.nrat_date 2020-04-02 Close