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Information × Registration Number 0212U005497, 0107U003341 , R & D reports Title The study of structure of Cytochrome P450 2E1 and it's active site popup.stage_title Head Chashchyn Mykola Oleksiyovych, Registration Date 30-11-2012 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 In this work we created accurate computer model of the spatial structure of cytochrome P450 2E1, which was used to study the molecular mechanisms of selective binding of the enzyme with ligands. We made theoretical models of specific interaction of cytochrome P450 2E1 with ligands, which were allowed to explore the topological characteristics of the active center of the enzyme. Conditions for heterologous expression, isolation and purification of recombinant functionally active cytochrome P450 2E1 were optimized. The amino acid residues important for selective binding of the active site of cytochrome P450 2E1 with substrates (Phe 298 and Thr 212) and inhibitors (Phe 106 and Phe 298) were found by using theoretical models. The computer models data of criticality of amino acid residues Phe 106, Phe 298 and Thr 212 for the functioning of cytochrome P450 2E1 were experimentally verified. These results have a great fundamental and practical importance for enzymology, pharmacology, diagnostics and medicine. Product Description popup.authors Заєць Володимир миколайович Кітам Володимир Олегович Максимчук Оксана василівна Росохацька Інна Василівна popup.nrat_date 2020-04-02 Close