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Information × Registration Number 0212U005547, 0110U000403 , R & D reports Title Investigation of reparative effect of cord blood low-molecular fraction on cryopreserved cells popup.stage_title Head Gulevsky Alexandr Kirillovich, Registration Date 12-12-2012 Organization Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine popup.description2 Purpose is to study the functional state of donated human blood leukocytes after low temperature storage in a medium containing low molecular weight cattle cord blood fraction (up to 5 kDa). Object of study: blood leukocyte concentrate human group A (II), harvested at the preservative "Glyugitsir." Methods: cryobiological, cytochemical, immunological, clinical, laboratory, differential centrifugation and erythrocyte sedimentation method dextran. The investigation of phagocytic activity of neutrophils after cryopreservation revealed stimulating effects of the cord blood fraction below 5 kDa and the comparator agent "Actovegin". The cord blood fraction below 5 kDa was shown to stimulate the engulfing and digesting capacities of donors' blood neutrophils at the concentration of 0.15 mg/ml, whereas "Actovegin" had a similar influence on neutrophils at the concentration of 1.50 mg/ml. The cord blood low-molecular fraction was established to be able to increase the level of production of reactive oxygen species by neutrophils after cryopreservation. The comparator agent "Actovegin" significantly affected the number of NBT-positive neutrophils after cryopreservation. The possibility of using the low-molecular fraction (below 5 kDa) from cattle cord blood or "Actovegin" in rehabilitating media for the purpose of reconstitution of functional activity of leukocytes, subjected to low-temperature storage, was proved. Product Description popup.authors Абакумова О.С. Гулевський О.К. Моісєєва Н.М. Нікольченко А.Ю. Трифонова Г.В. Щенявський І.Й. popup.nrat_date 2020-04-02 Close