1 documents found
Registration Number

0212U006461, 0112U001644 , R & D reports

Title

The molecular models of the complexes of biological compounds with nucleic acids at multimodal and competitive binding

popup.stage_title

Head

Shestopalova Anna Victorovna,

Registration Date

10-04-2012

Organization

Institute of Radiophisics and Electronics NASU

popup.description2

The main purpose of the research "Model" is a development of molecular models of complexes of biologically active substances (BAS) with nucleic acids in order to predict their biological activity at the molecular level. The development is based on the study of the physical parameters of complex formation in a multimodal and competitive binding of biologically active substances. The research was carried out by spectrophotometry, fluorescence and vibrational spectroscopy, differential scanning calorimetry (DSC), EHF dielectrometry, and computer simulation using the polynucleotides, DNA and biologically active substances (antitumor antibiotics and their newly synthesized analogues, mutagenes, and vitamins). New information on the types and structures of DNA - ligand complexes and their thermodynamic characteristics in multimodal and competitive binding taking into account the water environment was obtained. A new technique for calculating the parameters of ligand binding to the DNA according to the DSC was developed. The method for determining the effect of various substances on the structure of DNA was developed and patented. It allows to perform a preliminary assessment of the level of BAS mutagenicity. Online database ProtNA-ASA (www.protna.bio-page.org) was created. It provides systematic information about the conformational parameters and areas of accessible surface of atoms of nucleic acids in the protein-nucleic acid complexes.

Product Description

popup.authors

Березняк Катерина Германівна
Больбух Том Вікторович
Гасан Анатолій Йосипович
Кашпур Всеволод Андрійович
Круглова Олена Борисівна
Малєєв Володимир Якович
Семенов Михайло Олексійович

popup.nrat_date

2020-04-02

R & D report
The molecular models of the complexes of biological compounds with nucleic acids at multimodal and competitive binding
Head: Shestopalova Anna Victorovna. The molecular models of the complexes of biological compounds with nucleic acids at multimodal and competitive binding. (popup.stage: ). Institute of Radiophisics and Electronics NASU. № 0212U006461
1 documents found

Updated: 2025-05-11


Роздрукувати цю сторінку