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Information × Registration Number 0212U006705, 0109U007985 , R & D reports Title Development of technologies of the production and application of ecologically safe antiviral preparations for agriculture. Biosynthesis and study of microbial surface-active complexes. popup.stage_title Head Karpenko Olena Volodymyrivna, Registration Date 15-06-2012 Organization Physical Chemistry of Combustible Minerals Department Institute of Physical Organic Chemistry and Coal Chemistry of National Academy of Sciences of Ukraine popup.description2 Improved laboratory technology biosynthesis of surfactant produced by cultures of genus Pseudomonas. The conditions of cultivation of the strain Gordonia rubripertincta UKM Ac-122 for higher output biosurfactants. Biosurfactants received in the form of lipid extracts biocomplexes, the supernatant culture fluid. Analyzed their composition by thin-layer chromatography, spectrophotometry, determined the surface tension, CMD and emulguvalnu activity. With the help of mathematical modeling methods developed by the optimal parameters of the process of the microbial biosynthesis of surfactant. Established stimulatory effect of yeast extract (when added to the nutrient medium the growth of bacteria of genus Pseudomonas) on the yield of rhamnolipid biosurfactants. The analysis of samples biosurfactants that obtained by culturing the strain Pseudomonas sp. PS-17 on medium with glycerol and yeast extract. Investigated the synthesis of surfactant according to strain when using different sources of carbon and energy. Obtained preparations of surface-active glycolipids culture Pseudomonas fluorescens 8573 when grown on media with different carbon sources: mannitol, glycerin, sunflower oil and a mixture of glycerin - oil 1:1. Being upgraded laboratory technology for surface-active biocomplex and ramnolipids by combining the methods of concentrating the culture fluid, precipitation and extraction. Optimum conditions for biosynthesis of surfactant-based trehalozolipids strain Gordonia rubripertincta UCM Ac-122. Lipid extracts obtained from biomass, which include surface-active trehalozolipids. Carried out optimization of the nutrient media culture G. rubripertincta UCM Ac-122, namely, the determined concentration of carbon and nitrogen source for obtaining the maximum yield of biomass, biosurfactant and exopolysaccharides. With the help of a mathematical optimization was possible to increase biomass accumulation in 1,3 times, to increase the yield of cellular lipids in 1,9 times (from 4.35 to 8.12 g / l) and exopolysaccharides - a 5.1-fold (from 1.54 up to 7.89 g / l) compared with the control medium. According to the results of screening were selected promising producer of lipopeptides - Bacillus sp. F. Determined that this culture synthesizes biosurfactants on nutrient media with different nitrogen sources (NaNO3, NH4NO3), and under different conditions of cultivation (aeration, 220 rpm, aeration is limited). It is shown that the synthesis of lipopeptides can be stimulated by adding sodium citrate to the medium (4.0 g / l). The optimization of synthesis of biologics by mathematical modeling using analysis of variance. Carried out optimization of the culture medium of bacterial cultures of G. rubripertincta UCM Ac-122 and Pseudomonas sp. PS-17 : the content of carbon and nitrogen source, to obtain the maximum yield of biomass and biosurfactant. The optimal concentration of carbon and nitrogen source in nutrient medium for biomass accumulation, synthesis of cellular lipids and exopolysaccharides culture G. rubripertincta UCM Ac-122 using regression analysis and the method of ANOVA. Product Description popup.authors Вільданова-Марцишин Роза Іскандерівна Карпенко Олена Володимирівна Мідяна Галина Григорівна Пристай Мар'яна Володимирівна Щеглова Наталія Степанівна popup.nrat_date 2020-04-02 Close