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Information × Registration Number 0212U007399, 0112U005362 , R & D reports Title Creation of nano-sized supramolecular complexes to reveal and vizualize cancer stem cells popup.stage_title Head Goltsev Anatoliy Nikolaevich, Registration Date 05-06-2013 Organization Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine popup.description2 Research objects are cell cultures of Ehrlich carcinoma, bone marrow cells, nanoparticles of different geometrical forms based on orthovanadate. The research aim is to master the methods to visualize the stem normal and transformed progenitor cells using nanoparticles with different physical and chemical properties on the base of orthovanadate with following estimation of their genome profile. In the work there were used contemporary research methods: light and luminescent microscopy, electron transmission microcopy, spectrophotometry, flow cytometry, cell culturing in vivo. According to the designed methods there were synthesized aqueous colloid solutions of biocompatible nanoparticles of orthovanadates of spherical, spindle- and cord-like shapes. There have been studied physical-chemical and optical characteristics of the nanoparticles of different shapes and dimensions. The character of binding the nanomaterial with the transformed cells of Ehrlich carcinoma (EC), which is mammalian gland cancer of mice was studied. There was assessed the expression of nanog pluripotence gene in EC cells. There was shown the reduction of this index with the developing oncopathology. It has been established that the evidence of fluorescence when using spherical nanoparticles is first of all related to their physical properties, which are stipulated with their shape. In spite of the absence of fluorescence both of spindle- and cord-like nanoparticles are able to affect the functional activity of cancer stem cells of various differentiation degree, being in total population of EC. All types of nanoparticles not depending on the presence of fluorescence during their interaction with the cells cause the suppression of tumor growth in various extents. Comparative analysis of visualization of binding the fluorescence nanoparticles based on orthovanadate with normal and transformed progenitor cells has shown that the ability to penetrate inside cells is inherent only to spherical nanoparticles at both concentrations. Spindle-like nanoparticles at low concentrations had the most inhibiting effect on functional potential of transformed progenitor cells, moreover under the same concentrations there was found slight inhibition of the function of normal stem cells. For the first time there was shown the dependence of the effect of nanoparticles from their shape on the content of the amplification products of nanog self-maintenance gene transcripts in EC cells and the absence of their effect on hemopoietic progenitor cells of bone marrow. The performed studies have proved the possibility of using nanoparticles of certain shape and concentration to visualize and inhibit the growth of EC, which can be used in diagnostics and treatment of oncological diseases. Product Description popup.authors Бабенко Наталья Миколаївна Бондарович Микола Олександрович Гаєвська Юлія Олександрівна Дубрава Тетяна Георгіївна Луценко Олена Дмитрівна Останков Максим Вадимович Останкова Людмила Василівна Сафранчук Ольга Володимирівна Челомбитько Ольга Василівна Ямпольска К.Є popup.nrat_date 2020-04-02 Close