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Information × Registration Number 0212U008720, 0112U005014 , R & D reports Title Molecular mechanisms of substrate specificity in cytochrome P450 mediated reactions popup.stage_title Head Chashchyn Mykola Oleksiyovych, Registration Date 18-02-2013 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 The aim of the second phase of the work was to develop a theoretical model of the interaction of ligands with membrane-associated full-length cytochrome P450 2E1 and to study the molecular mechanisms of its substrate specificity. During performance of the phase 2 of the project areas for specific binding with substrates (Phe298, Ala299 and heme) and inhibitors (Phe106, Leu210, Thr212, Leu215, Gln216, Val364 and Phe478) in the active site of the enzyme were identified for the first time. Modeling of the cytochrome P450 2E1 spatial structure and identification of main characteristics of its interaction with ligands made it possible to characterize the topology of the enzyme active site and to facilitate understanding of mechanisms of its functioning. Product Description popup.authors Заєць Володимир Миколайович Кітам Володимир Олегович Максимчук Оксана Василівна Росохацька Інна Василівна Рущак Володимир Володимирович popup.nrat_date 2020-04-02 Close