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Information × Registration Number 0212U009016, 0112U005239 , R & D reports Title Role of the IRE-1a signaling pathways in glioblastoma progression: evaluation as a therapeutic targets for antitumor drug design. popup.stage_title Head Minchenko O.H., Registration Date 26-12-2012 Organization A.V.Palladin Institute of Biochemistry of National Academy of Sciences popup.description2 Results and development of this investigation shown that the expression level of E2F8, HSPB8 and PBEF mRNA strongly decreased at switch off the ERN1 sensor and signaling enzyme function and correlated with suppression of tumor growth from these cells. Short interfering RNA (siRNAs) for E2F8, HSPB8 and PBEF, transfected into U87 glioma cells, suppress the level of E2F8, HSPB8 and PBEF by qPCR, besides that siRNA-PBEF suppresses the expression not only PBEF mRNA, but also mRNA expressions of many key genes which control cell proliferation and malignant tumor growth. Thus, 5-fold suppression of PBEF mRNA expression by PBEF-siRNA activates the expression of BIRC5, RAB5C, TSPAN13, HOMER and IRS1 genes and suppresses the expression of KRT18, PER2, PRKCA, MYRL2, CLU, CUL4B and DEK genes. This data shown tight connection between gene expressions and open new possibility to find optimal ways for affecting the tumor growth. Product Description popup.authors Божко Ірина Володимирівна Гармаш Яна Анатоліївна Кубайчук Катерина Ігорівна Мінченко Дмитро Олександрович Мінченко Олександр Григорович popup.nrat_date 2020-04-02 Close