Search academic texts

Information × Registration Number 0213U000012, 0112U004302 , R & D reports Title The role of genetic and epigenetic alterations of histone deacetilases activity in regulation of lifespan-associated genes expression in Drosophila melanogaster popup.stage_title Head Vayserman Aleksandr Michailovich, Registration Date 09-01-2013 Organization State Institution "Institute of Gerontology AMS Ukraine" popup.description2 It was suggested that because of the impact of histone deacetylase inhibitor sodium butyrate (SB) on epigenetic control of gene function and therefore possibility of long-term effects, SB supplementation during the larval stage of development only may also increase adult life span (LS). Experimental validation of this prediction demonstrated that nutritional supplementation with SB during the larval stage at a concentration of 20 mmol/l resulted in a significant increase in the male mean LS; maximum LS was significantly increased in males treated with SB at concentrations of 10, 20 and 40 mmol/l. Female mean LS was unchanged following the SB administration; maximum LS was significantly increased in female group treated with SB at concentration of 10 mmol/l only. Female reproductive activity was the same in all groups. We hypothesized that the observed long-term effect of SB exposure on the flies' longevity could be caused by the induction of persistent epigenetic changes. To test this hypothesis, the levels of expression of the longevity-associated genes (hsp70, sir2 and InR) were determined in insects fed with different concentrations of SB during development and kept under both normal and stressful (heat shock and starvation) conditions during adulthood. The expression level of sir2 gene, known to mediate longevity in the fly through a pathway related to calorie restriction, in the group treated at the larval stage with 20 mmol/l SB was significantly higher after the stress (starvation) than in the control group. Product Description popup.authors Вайсерман Олександр Михайлович Коляда Олександр Костянтинович Кошель Наталія Михайлівна Орехова Лідія Вадимівна popup.nrat_date 2020-04-02 Close