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Information × Registration Number 0213U000281, 0112U003505 , R & D reports Title Investigation of ultrastructural features of marrow stromal cells in vitro at decrease of gravitation loading (space experiment "Oblast") popup.stage_title Head Rodionova Natalia Vasilievna, Registration Date 29-01-2013 Organization Schmalhausen Institute of Zoology NAS of Ukraine popup.description2 The report consists of 30 pages, including 13 illustrations (light and electron micrographs), 1 table, 20 sources of literature. The object of study - the culture of stromal cells in the bone marrow of rats ex vivo, in vitro. Purpose - to examine the in vitro characteristics of colony, cytological and ultrastructural features, differential and osteogenetical potential bone marrow stromal cells by removing the gravity load. Research methods - cell culture ex vivo and in vitro, light microscopy, electron microscopy, digital photography, morphometric and statistical analysis using computer programs. Results and novelty: in experiments on rats with the removal of the reference loading on the hind limbs (a period of 1.5 months) to obtain new data for science about sensitivity to gravity bone marrow stromal cells containing MSCs and osteogenic progenitor cells. Found that in the simulated microgravity decreases the number of colony-forming bone marrow stromal cells, reducing their differential, including osteoblastic potency, the induction adipocytogenesis. This is confirmed by electron microscopy studies. Research results are of fundamental importance, bring new understanding of the cellular mechanisms of gravitation sensitivity bone, and can be used to develop test systems for the diagnosis and prediction in traumatology and orthopedics, space medicine. Keywords: stromal cells of the bone marrow, stromal progenitor cells, cell culture ex vivo, colony formation, cell differentiation, microgravity simulation, light microscopy, electron microscopy. Product Description popup.authors Бабак Світлана Віталіївна Родіонова Наталія Василівна Скрипченко Олена Володимирівна popup.nrat_date 2020-04-02 Close