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Information × Registration Number 0213U000537, 0110U002202 , R & D reports Title To study efficiency of antineoplastic action of complex of magnetic micro- and nanoparticles and doxorubicin at an electromagnetic irradiation in an experiment popup.stage_title Head Schepotin I.B, Registration Date 14-02-2013 Organization Ukrainian Research Institute of Oncology and Radiology popup.description2 Object of study – magneto-sensitive complex micro- and nanoparticles of Fe3O4 with KCl, doxorubicin, cell lines of mice: LL Lewis lung carcinoma, non-small cell lung cancer A-549 human and animal tumors: carcinoma Guerin, carcinoma Guerin resistance to doxorubicin, carcinosarcoma Walker-256 and Lewis lung carcinoma. Equipment - mechanomagnetic reactor, the apparatus for local short-wave inductothermy "Mahniterm" (Radmir, Ukraine); mercury thermometer S195 (Medisana AG, Hilden, Germany); diving rule metal length 300 mm, scanning electron microscope JEOL JSM-6490LV (Japan); chemiluminometr HLM1TS-01 (Ukraine), spectrophotometer NanoDrop 1000 («Thermo Scientific», USA), spectroscope RE1307 (ZEPS AN), centrifuge OPN-3, microcentrifuge MICKROSPIN FN-2400, magnetometer «Vibrating Magnetometer 7404 VSM» («Lake Shore Cryotronics, Inc. », USA), thermostat TS-80M2. Aim - to examine the efficacy of antitumor action of the complex magnetic micro-and nanoparticles and doxorubicin during electromagnetic radiation in experimental models of tumor. Methods – physicotechnical, physicochemical, laboratory and statistical. The design and produce mechanomagnetic reactor for mehanomagnetochemical synthesized multifunctional complex based on joint effect of mechanochemical activation and electromagnetic radiation. The developed of multifunctional magneto-sensitive complex with paramagnetic doxorubicin and bioengineering technology for its targeted delivery to malignant tumors. The method of scintigraphy directly visualized accumulation multifunctional magneto-sensitive complex synthesized from radiopharmaceuticals 99mTc-pirfotecuh in carcinosarcoma Walker-256. In experimental models of tumor: carcinoma Guerin, carcinoma Guerin resistance to doxorubicin and Lewis lung carcinoma showed that the effectiveness of anticancer magneto-sensitive complex and radiofrequency hyperthermia was on average 26% greater than the effect of conventional doxorubicin, according to calculations of tumor volume on the last up period of growth. Experimentally confirmed indirect role of topoisomerase II in antitumor effects of doxorubicin under the influence of electromagnetic radiation. Change content of hemo and non-hemo iron with nitric oxide activity FeS-protein in elektron- transport chain of mitochondria are most pronounced in tumor tissues and liver of animals after administration of magneto-sensitive complex and radiofrequency hyperthermia than conventional doxorubicin. The data showing that magneto-sensitive complex and radiofrequency hyperthermia initiation more pronounced morphological changes in the mitochondria of cells of Lewis lung carcinoma than conventional doxorubicin. A mathematical analysis and computer model of antitumor effects of magneto-sensitive complex and radiofrequency hyperthermia for malignant tumors that can formalize several tasks targeted delivery and initiation of free radical activity of the drug. Field of application - Oncology. MALIGNANT TUMORS, NANOPARTICLES, MAGNETO SENSITIVE COCOMPLEX, RADIOFREQUENCY HYPERTHERMIA. Product Description popup.authors Бездєнєжних Н.О. Дзятковська І.І. Дзятковська Н.М. Крючков Є.І. Ніколов М.О. Орел В.Е. Рихальський О.Ю. Романов А.В. popup.nrat_date 2020-04-02 Close