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Information × Registration Number 0213U000833, 0108U001671 , R & D reports Title Investigate of influence mesenhymal and embryonic cells for character of reparftive processes in the tissues were changed pathological of animals organism. popup.stage_title Head Mazurkevich Anatoliy, Registration Date 01-04-2013 Organization Ukrainian Education and Research Institute of Bioresearches Quality and Life Safety. popup.description2 Object of research - embryonic stem cells, mesenchymal stem cells and embryonic fibroblasts in the stimulation of reparative processes in the skin of animal- recipients. Objective: To improve the methods of obtaining of pigs embryos in vitro for obtaining the inner cell mass of embryos; to investigate the suitability of gelatin biomatrix of different concentrations for immobilization of mesenchymal stem cells in rats and dogs, to explore the effect of immobilized allogeneic mesenchymal stem cells and embryonic fibroblasts on the course of reparative processes in the healing of skin's wound defects in rats; to examine of indicators of immune compatibility of transplanted allogeneic and xenogeneic mesenchymal stem cells and allogeneic fibroblasts in animals-recipient; to conduct the directed differentiation of mesenchymal stem cells into osteogenic and chondrogenic directions; to investigate the functional phenotype characteristics of uncommited mesenchymal stem cells of different species during cultivation in vitro; to study the ability of uncommited mesenchymal stem cells of rats, rabbits and dogs through expression of different functional molecules to acquire the specific functional phenotype under appropriate conditions. Methods of exploration - surgical, mathematical, histological, immunocytochemical, immunological, clinical, statistical. During the explorations: the methods of obtaining of pigs embryos in vitro for obtaining the inner cell mass of embryos were worked out and improved, the effect of allogeneic mesenchymal stem cells and embryonic fibroblasts on the course of reparative processes in the healing of skin's wound defects in rats was explored; the features of immune compatibility of allogeneic mesenchymal stem cells and embryonic fibroblasts of rats after local application for correction of reparative processes in the skin of rats and allogeneic mesenchymal stem cells, embryonic fibroblasts and xenogeneic (rabbit MSCs) mesenchymal stem cells after their introduction into the bloodstream were established. CD receptor apparatus of uncommited mesenchymal stem cells of different species of animals at different passages was investigated. The ability of uncommited mesenchymal stem cells of animals to differentiation during in vitro cultivation was explired. Areas of using - veterinary medicine. Product Description popup.authors Бобось Оександр Леонідович Голяков Олег Юрійович Данілов Василь Бенедиктович Журба Віталіна Ігорівна Журенко Олена Василівна Золтан Надія Іванівна Карповська Віра Миколаївна Ковпак Віталій Васильович Мазуркевич Анатолій Йосипович Малюк Микола Олексійович Харкевич Юрій Олександрович popup.nrat_date 2020-04-02 Close