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Information × Registration Number 0213U000871, 0110U000299 , R & D reports Title Comparative toxicity of lead micro- and nanoparticles in vitro and in vivo experiments (towards the improvement of tocixological and hygienic methods of studying heavy metals) popup.stage_title Head Isaac Trachtenberg, Доктор медичних наук Registration Date 09-04-2013 Organization State Institution "Institute for Occupational Health of the Academy of Medical Scieces of Ukraine" popup.description2 In vitro and in vivo studies were performed to determine the characteristics of the toxic effect of lead nitrate (microform) and lead sulfide (nanoparticles PbS). In vitro experiments revealed that the PbS nanoparticles, regardless of size showed a greater cytotoxic effect on cell culture, a more pronounced membranotoxic effect to red blood cells and blood plasma protein denaturation comparing to lead nitrate microparticles. In vivo experiments established differences in lead toxicokinetics and toxicodynamics. Lead nitrate exposure caused metal accumulation in the liver and kidneys, the imbalance of trace elements (Fe, Zn, Mg, Ca). In post-exposure period lead elimination was slower after the exposure to nanoparticles of PbS. The disturbances of iron metabolism, iron accumulation in the liver were more significant after the introduction of lead sulfide nanoparticles. Development of lead intoxication was accompanied by a violation of heme synthesis, decreased hemoglobin levels. Changes in blood coagulometric characteristics in form of hypocoagulation were most important in case of lead nitrate. After post-exposure period there was a significant increase in the level of cholesterol, triglycerides and в-lipids after PbS exposure, which may indicate atherogenic effect of lead. Effect of lead on the immune system was characterized by an increase in the relative weight of immune organs (thymus, spleen), stimulation of the immune response, especially under the influence of lead sulfide. PbS nanoparticles actively promoted the absorption activity of phagocytes and oxidative stress in it, decrease of cerculating immune complexes (CIC) in blood, which may indicate the influence of nanoparticles on Ig structure and adsorption of CIC . It should be noted that the general toxic properties were more characteristic for lead microparticles, although nanoparticles showed more effect on cells membrane and metabolic processes. Product Description popup.authors І.В. Губар І.О. Мележик К.П. Козлов Л.А. Легкоступ Л.М. Зайцева Л.М. Краснокутська М.Л. Марченко М.М. Коршун Н.М. Дмитруха О.Л. Апихтіна О.С. Лагутіна Т.К. Короленко popup.nrat_date 2020-04-02 Close