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Information × Registration Number 0213U001064, 0110U000237 , R & D reports Title Determination of cell effects of combined influence of heavy metals, ionizing and nonionizing radiation and possibility of their correction by polymineral natural substances popup.stage_title Head Lavrenchuk Halina, Registration Date 11-01-2013 Organization State institution "National research centеr for radiation medicine of the National academy of medical sciences of Ukraine " popup.description2 Objective: To investigate the biological effects in cells under combined influence of agents of chemical and physical nature - heavy metals ions, ionizing and non-thermal electromagnetic radiation of low intensity, to establish the character and impact of modifying influence of some heavy metal ions on radiogenic effects in cells and identify opportunities to minimize the negative effects by polymineral complexes of natural origin. Object of study: growth kinetics, mitotic activity, apoptosis, energy enzymes in the cell culture L929. Research methods: cultivation of inoculated cells, cytological (cultivation and analysis of cell culture by optical microscopy), cytochemical (assessment of mitochondrial enzymes activity), biophysical (flow cytometry), statistics. Investigation of heavy metal ions (Pb +2, Cu +2, Ni +2 and Cr +2) influence in the concentration range of 0.01 - 10 mmol / l on the kinetics of growth, proliferative and mitotic activity of cells in vitro allowed us to establish not only cytotoxic effect on cell viability, but also a stimulating effect (eg Cr +2) in the range of ions low concentrations. The study of apoptosis in cell cultures by incubation with the studied trace elements showed that apoptosis is induced by all trace elements, but in different concentrations. Cytochemical studies showed that heavy metal ions alter the activity of mitochondrial energy enzymes, which indicates the compensatory nature of these changes. In terms of survival in monolayer cultures of cell line L929 the average effective dose (SE50) for each DOE was calculated and a number of cytotoxicity was built, indicating the high toxicity of nickel and lead. Study of heavy metal ions influence on the viability of cells exposed to ionizing radiation allowed us to set polymodal character changes. Lead ions enhance radiation effects, lead to dose-dependent dystrophic-destructive changes and induce apoptosis in cell culture. Nickel ions through their genotoxic properties significantly reduce the mitotic activity and increase apoptosis in cultured cells irradiated at doses of 0.5 and 5 Gy; in sublethal doses exhibit a dominant influence on the survival and proliferative activity of cells. Trace elements chromium and copper in concentrations 0.01 - 10 mmol / l did not exhibit cytotoxic properties in cell cultures irradiated at doses of 0.5 and 5 Gy, but the damaging effect of sublethal doses of 10 Gy does not depend on the concentration of these elements: the number polycariocytes increases in 25 (Cu +2) - 40 (Cr +3) times compared to intact control. Number of apoptotic cells under combined influence of radiation and ions of copper or chromium does not depend on the dose of radiation, nor of their content in cell cultures. Additional effects of electromagnetic millimeter waves on cells irradiated in the presence of heavy metals showed features of cellular reactions. When exposed to radiation, incubation of cells with lead ions protective effects of electromagnetic radiation occurs only for radiation doses of 0.5 and 5 Gy and for low concentrations of elements 0.01 - 1 mmol / l. When incubating irradiated cells with ions of nickel, copper and chromium the modifying influence of electromagnetic radiation was not found. Cytochemical studies showed that increased energy exchange in irradiated cells through activation of glycolysis is common to all heavy metal. Only nickel ions induced increase of succinate dehydrogenase activity in the concentration range 0.01 - 0.1 mmol / l. Application of polymineral substances "Minerol" and "Benta" reduced the negative effects of ionizing radiation and heavy metal ions, increased survival, proliferative and mitotic activity. According to experimental results a complex polymodal character of changes during apoptosis and in the system of energy exchange was shown. Product Description popup.authors Асмолкова Валентина Сергіївна Гапєєнко Дар'я Дмитрівна Оксамитний Віктор Миколайович Олейнікова Тетяна Георгіївна Чоботько Григорій Михайлович popup.nrat_date 2020-04-02 Close