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Information × Registration Number 0213U001070, 0112U002408 , R & D reports Title Investigation of possible persistence of hidden chromosome instability induced by ionizing radiation exposure in human somatic cells. popup.stage_title Head Pilinskaya Maria, Доктор медичних наук Registration Date 11-01-2013 Organization Research Centre for Radiation Medicine popup.description2 Object of study - the frequency of chromosomе aberrations in peripheral blood lymphocytes of healthy individuals who denied the conscious contact with mutagenic factors. The subject of the study - the hidden chromosome instability in individuals from the comparison group in the short- and long- term cultures of peripheral blood lymphocytes. Purpose - to examine the hidden chromosomal instability in peripheral blood lymphocytes of unexposed individuals from the comparison group and its persistence over time. In accordance with the objectives of the work of voluntary cytogenetic survey formed the intact comparison group of apparently healthy individuals, residents of Kiev, who denied the conscious contact with known or potential mutagens (including ionizing radiation) - 15 people (all male) aged 29 to 54 years the average age - 40 years. To assess the possible persistence of hidden chromosomal instability in somatic cells of healthy donors from the comparison group used two previously improved by us test systems: «G2-bleomycin sensitivity assay» and two-termed - short- and long- time (over 48 and 100 h., respectively) culturing of peripheral blood lymphocytes Found that the background (the original) frequency of chromosome aberrations in peripheral blood lymphocytes of individuals from the comparison group corresponded to the level of spontaneous somatic chromosome mutagenesis and did not differ significantly in terms of both cell cultures. Showed that in the short-term lymphocyte cultures exposed to bleomycin significantly increased both individual and mean-group frequencies of chromosome aberrations with considerable inter-individual variations, independent of baseline cytogenetic parameters. Found that in the long-term cultures of lymphocytes with bleomycin in all examined individuals significantly reduced the frequency of chromosomt aberrations as compared with the first mitosis, thus significantly reduced not only the mean-group cytogenetic effect, but addition to the background level of chromosomal aberrations, suggesting the gradual elimination of aberrant cells in successive mitosis. Total for the period 29 150 metaphases analyzed, of which 14 950 - without mutagenic load , 14 200 - with bleomycin exposure. Scope - radiation genetics, oncology, occupational medicine. The degree of implementation - 5 articles, 4 abstracts. Product Description popup.authors Єрємеева М.Н. Дибська О.Б. Дибський С.С. Степанова Л.С. popup.nrat_date 2020-04-02 Close