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Information × Registration Number 0213U001855, 0110U002207 , R & D reports Title To study features of the biological response of an organism in dynamics of interferon therapy at skin melanoma patients and to determine immunological criteria of efficiency of the combined treatment popup.stage_title Head Filchakov F.V., Registration Date 21-02-2013 Organization Ukrainian Research Institute of Oncology and Radiology popup.description2 Object of research: immunocompetent cells, аutoantibodies. The purpose - To determine features of shifts in immune system of patients skin melanoma under influence interferon therapy for forecasting efficiency of the combined treatment. Methods of research - immunological, flow cytometry, a method of cell culture, statistical. The equipment: centrifuges ОPN-3; a microscope; flowing cytometer FACScan; termostates; the СО2-incubator. Clinical and immunological investigation of 27 patients with skin melanoma with metastasises in regional lymph nodes of a ІІІВ-ІІІС stage in dynamics of the combined treatment with application of different interferon therapy regimes has been studied. It is established, that carrying out of induction IFN-alpha2b therapy irrespective of the regimen of application (up to or after surgical treatment) is accompanied by development of the leukocytopenia. Induction IFN-alpha2b therapy in neoаdjuvant regimen promotes restoration after surgical treatment of quantity T-lymphocytes, but does not liquidate increased Тrеg contents. The similar rate in аdjuvant regimen does not restore of decreased quantity T-lymphocytes, but promotes reduction of Тrеg level in circulation. Induction IFN-alpha2b therapy both in neoаdjuvant, and in аdjuvant regimen, promotes reduction in peripheral blood of patients of increased contents of activated lymphocytes (HLA-DR+ and CD95+). In case of application of induction and supporting rates IFN-alpha2b therapy in аdjuvant regimen increased relative quantity CD4+25+-lymphocytes also is reduced. As against it at reception by patients in аdjuvant regimen only a supporting rate - relative quantity CD4+25+-lymphocytes stably high during. Induction IFN-alpha2b therapy in neoаdjuvant regimen (not in аdjuvant) results in decrease of cytotoxic lymphocytes activity, and promotes restoration of the answer lymphocytes on mitogen. Level ТSH remains within the limits of norm, and concentration TPO-Ab and ТG-Аb decreases after 3 months supporting IFN-alpha2b therapy. SKIN MELANOMA, INTERFERON THERAPY, LYMPHOCYTES, IMMUNOPHENOTYPE, CYTOTOXIC ACTIVITY, PROLIFERATION LYMPHOCYTES Product Description popup.authors Ільченко Ю.М. Кукушкіна С.М. Льон Г.Д. Шуміліна К.С. popup.nrat_date 2020-04-02 Close