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Information × Registration Number 0213U001869, 0110U001414 , R & D reports Title Immunobiological characteristic of separated fractions of diphtheria and tetanus toxoids and determination of modes of exotoxins modification by physicochemical methods popup.stage_title Head Babych Evgenij Mihailovich, Доктор технічних наук Registration Date 25-02-2013 Organization The Mechnikov Research Institute of microbiology and immunology at Kharciv popup.description2 Object of study: diphtheria and tetanus toxins and toxoids, derivatives of diphtheria toxin. The aim of study: to develop scientific approaches to obtaining of safer diphtheria and tetanus toxoids without unprotective fractions and toxic modifiers. Methods of investigation: microbiological, immunological, biological, chromatographic, statistical. It was found experimentally that purified industrial tetanus toxoid contained ballast proteins, which might be shed by certain modes of ultrasonic treatment. The possibility of obtaining of diphtheria toxoid using chemical and physicochemical factors was studied. It was proved that the influence of the physical factor did not cause destruction or inactivation of native diphtheria toxin (DT). It was determined that twofold reduction of formalin percentage did not significantly affect the specific activity of obtained DT derivatives and DT derivative obtained by physicochemical factors was safe. It was established that the toxoiding process could be controlled by fluorometry. The catabolic activity of microorganisms was studied by the amount of oxidized glucose. Determination of catabolic activity of microorganisms makes it possible to conduct selective search of strains and evaluate epidemic situation. Product Description popup.authors Антушева Тетяна Іванівна Бабич Євгеній Михайлович Ждамарова Лариса Анатолівна Калініченко Світлана Вікторівна Рижкова Тетяна Анатолівна Скляр Надія Іванівна popup.nrat_date 2020-04-02 Close