Search academic texts

Information × Registration Number 0213U001985, 0112U008037 , R & D reports Title Study of the participation of intercellular interactions in the immune system in the development of stress reactions popup.stage_title Head Ostapshenko L.I., Registration Date 20-03-2013 Organization Taras Shevchenko Kiev university popup.description2 Thymus-derived cell cultures of intact animals, post-acute and post-prolonged stress mice, as well as post-prolonged stress mice, treated with thymic MSCs or fetal MSCs, have been isolated. Stomach-derived MSCs cultures differed by slow growth. Gastric fibroblasts were morphologically different from thymic fibroblasts by smaller size. In contrast to cultures of thymic MSCs, in cultures of gastric MSCs the epitheloid colonies were determined at the beginning, and then the fibroblastoid colonies appeared. The highest number of individual fibroblastoid cells and colonies were noted in cultures derived from the mice stomach after prolonged stress and thymic MSCs injection. Prolonged stress significantly reduces the weight of the thymus and thymic index with a tendency to the thymocyte absolute number and thymus cellularity decreasing. Prolonged stress significantly increases the splenic index with a tendency to spleen weight increasing. At the same time, a decrease in the relative number of lymphocytes in the peripheral blood was observed, but bone marrow cellularity wasn't changed. Alteration in cellular composition of the immune system organs at prolonged stress was combined with significant suppression of antibody formation and with tendency to the delayed-type hypersensitivity reducing. The injection of thymic MSCs or fetal MSCs at prolonged stress development leads to cellular composition normalization of the immune system organs, the antibody formation and the delayed-type hypersensitivity reaction, which opts for the cell therapy with MSCs use as promising treatment for prolonged repetitive stress reactions in the future. Product Description popup.authors Калініченко О.В. Філенко О.В. Чех Д.Л. popup.nrat_date 2020-04-02 Close