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Information × Registration Number 0213U002054, 0110U006124 , R & D reports Title Developing a fundamental basis for obtaining new generation immunoreagents popup.stage_title Head Okunev Oleg Volodymyrovych, Registration Date 14-01-2013 Organization Institute of Molecular Biology and Genetics popup.description2 We aimed construction of the chimeric protein scFv-BАРmut which comprises single-chain antibody (single-chain fragment variable, scFv) and alkaline phosphatase moieties (bacterial alkaline phosphatase mutated, BАРmut) produced by genetically engineered strain of Escherichia coli and /or fluorescent proteins EGFP та mCherry, as well, as their obtaining by bacterial synthesis and characteristics. Proteins produced by P. pastoris namely scFv(IFNа2b)-APmut, scFv(IFNа2b)-EGFP, scFv(CD34)-EGFP та scFv(IFNа2b)-mCherry were obtained. Their molecular biological and biochemical characteristics were determined. The opportunity of application such immunoreagents for immunodiagnostics was shown. Obtained chimerical molecules of antibodies with alkaline phosphatase, fluorescent proteins are high sensitive molecular probes and perspective candidates for different types of immunochemical analysis (ELISA, western-blot, immunocytochemistry). The specificity and sensitivity of antigen detectio n by obtained immunoconjugates are not inferior to existing commercial antibodies.5481 Product Description popup.authors Окунєв О.В. Павлова М.В. Шпильова С.П. popup.nrat_date 2020-04-02 Close