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Information × Registration Number 0213U002058, 0112U003749 , R & D reports Title Clathrochelates of transition metals as inhibitors of some enzymes of nucleic acids biosynthesis system and prospective antitumor and antiviral pharmaceuticals. popup.stage_title Head Dubey Igor, Доктор хімічних наук Registration Date 14-01-2013 Organization Institute of molecular biology & genetics popup.description2 Biological activity of clathrochelates was for the first time studied on cell models in vitro. The complexes neither affected the infectivity of tobacco mosaic virus (U1 strain) nor demonstrated the bacteriostatic effect against E.coli, B.subtilis and C.albicans. At the same time, a number of compounds demonstrated antitumor activity at micromolar concentrations. Mechanisms of biological activity of the complexes were studied, first of all their unusually high activity in transcription reaction (found at the previous stage of the project). Electrophoretic method Mobility Shift demonstrated that clathrochelates do not bind efficiently to DNA. Kinetic experiments in the transcription system in vitro allow suggesting that active inhibitorsact via the interaction with RNA polymerase - DNA template complex. These data were confirmed by the results of molecular modeling (docking). Product Description popup.authors Ільченко М.М. Дубей Л.В. Костенко О.М. Криворотенко Д.В. Кузів Я.Б. Негруцька В.В. popup.nrat_date 2020-04-02 Close