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Information × Registration Number 0213U002125, 0112U004176 , R & D reports Title Investigation of microgravity biological effects at the membrane and cellular levels ("Biolaboratory-M") popup.stage_title Head Kordyum Yelizaveta Lvovna, Registration Date 15-01-2013 Organization M.G.Kholodny Institute of Botany popup.description2 The data of studies of the effect of simulated microgravity on: 1) the organization of the cytoskeleton in Arabidopsis thaliana root cells and gene expression of proteins associated with tubulin microtubules and actin microfilaments (clinorotation) and 2) the ultrastructure, differentiation and functional potency of bone marrow stromal cells femur and tibia of white rats that have osteogenic precursor cells (removal of bearing on the hind limbs of animals by hanging tails at 35 *) using modern methods of light, electron and confocal microscopy, biochemistry (electrophoresis) and molecular biology (PCR), statistics, inhibition analysis. Method was developed for obtaining preparations of the cytoplasmic membrane of plant cells in culture in vitro for conservation at -15 * C for further study of its physical and chemical properties in conditions of real and simulated microgravity. Completed experiments are a necessary step for the realization of space experiments "Membrane", "Cytoskeleton", "Oblast" in the "Biolaboratories-M" on board of the RS ISS. Current problems for further studies of the effect of microgravity on the cellular and molecular level were identified. Product Description popup.authors Артеменко Ольга Анатолієвна Бабак Світлана Віталієвна Заславський Віктор Абрамович Климчук Дмитро Олександрович Кордюм Єлизавета Львівна Родіонова Наталія Васильйовна Скрипченко Олена Володимирівна Шевченко Галина Валерієвна popup.nrat_date 2020-04-02 Close