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Information × Registration Number 0213U002161, 0110U005761 , R & D reports Title The research of genetic backgrounds and influence of environmental factors on realization of genetic predisposition to cancer development popup.stage_title Head Buchynska Lyubov Georgiyivna, Доктор біологічних наук Registration Date 16-01-2013 Organization R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology popup.description2 Among cancer cases, 30% endometrial cancer (EC) patients and 23% ovarian cancer (OC) patients reported a family history of cancer. Assessment of DNA damage in lymphocytes was performed by DNA comet assay. DNA damage were quantified as the comet tail moment (TM). The difference between the basal tail moments of peripheral blood lymphocytes (PBL) in endometrial and ovarian cancer patients with family history of cancer didn't alter significantly from patients with sporadic EC and OC. Among the endometrial cancer patients the sensitivity of PBL to bleomycin was higher in patients with family history of cancer (TM 104,95 ± 1,16) in comparison with patients without family history of cancer (TM 97,32 ± 0,62). It has been revealed that the efficiency of DNA repair depended on family history of cancer in EC and OC patients. After repair, PBL restored the damaged DNA to the level of tail moment 55,00±2,43 in EC patients with family history of cancer and up to 22,77±1,13 in patients with sporadic cancer. Among the OC patients TM was higher in patients with family history of cancer (69.27±3.95) in comparison with TM of patients without family history of cancer (16.94±1.59). Our results indicate that PBL of EC patients with family history of cancer are characterized by hypersensitivity to mutagenic factors and deficient DNA repair. In lymphocytes of OC patients with family history of cancer low DNA repair capacity is occurred. Product Description popup.authors Білик Олена Олександрівна Брєєва Ольга Володимирівна Буднікова Дарія Володимирівна Бучинська Любов Георгієвна Глущенко Надія Миколаївна Дьоміна Емілія Анатоліївна Несіна Ірина Петрівна Юрченко Наталія Петрівна popup.nrat_date 2020-04-02 Close